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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 322 results for: nans

Showing: 161 - 180 of 322 results

  1. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...doi.org/10.1371/journal.pone.0076310 Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa...Nonhuman Primates after Intramuscular Injection of Recombinant Adeno-Associated Virus. Journal of Virology, ...

  2. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...then be exposed to a lysis buffer to release recombinant proteins directly onto the nitrocellulose for... 1 M NaCl in order to enhance folding of the recombinant proteins. Optimal salt concentration will vary...

  3. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...from multiple primer pairs, one can infer the predominant state of registers within a sample using a metric...states in a sample; instead, it will suggest the predominant state of registers in a sample based on a similarity...

  4. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...of dsRNA. In plants, CRISPR technologies are predominantly used to disrupt function at the level of DNA...Interference System in Physcomitrella patens Reveals a Dominant Role of Augmin in Phragmoplast Microtubule Generation...

  5. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...non-mutant line but no signal in the mutant line! Recombinant Strategies This approach is essentially the opposite...ready-to-use antibodies from Addgene Designing a recombinant validation experiment? Find plasmids here!  ...

  6. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...Zolotukhin S, Reier PJ, Mandel RJ, Muzyczka N (2004) Recombinant AAV Viral Vectors Pseudotyped with Viral Capsids... Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations from Viral...

  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...more reproducible/renewable reagents, such as recombinant or hybridoma-derived monoclonal antibodies. It...µg/mL (1/100). * = monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al., ...

  8. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...including indels. Therefore, they engineered a dominant-negative mutant of the protein MLH1, a component...prime editing systems by manipulating cellular determinants of editing outcomes. Cell, 184(22), 5635-5652...

  9. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from... plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids...

  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...phosphatase-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-...tool for use as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime...

  11. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...AAV9 in the liver. AAV9-X1.1 effectively and predominantly transduces neurons across brain regions, with...throughout the non-human primate brain. Nature Nanotechnology, 1–11. https://doi.org/10.1038/s41565-023-01419...

  12. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ...latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies."  Hot tip! This protocol can easily...

  13. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th day of CRISPR...

  15. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...giant paper mache Krampus performing routine lab maintenance tasks. Hot tip: make sure your PI/facilities ...

  17. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...Instagram all summer long to see what kind of shenanigans we get into!  We hope you are as excited as we...
Showing: 161 - 180 of 322 results