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We narrowed to 326 results for: nans

Showing: 161 - 180 of 326 results
  1. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... scFvs. What is an scFv? scFVs are a type of recombinant antibody. They are ~25 kDa single polypeptides...coli Expression System in Producing Antibody Recombinant Fragments. IJMS 21:6324 . https://doi.org/10.3390...
  2. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...endogenous epitope tags, or the introduction of dominant mutations of a gene into the endogenous allele...generating human somatic cell gene knockouts using recombinant adeno‐associated viruses." Nucleic acids research...
  3. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...glnV44 λ- JM109   General cloning and plasmid maintenance, blue/white screening, partly restriction-deficient...(mrr-hsdRMS-mcrBC) Pir1    For cloning and maintenance of a plasmids with R6Kγ origin; contains a mutant...
  4. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...doi.org/10.1371/journal.pone.0076310 Damdindorj, L., Karnan, S., Ota, A., Hossain, E., Konishi, Y., Hosokawa...Nonhuman Primates after Intramuscular Injection of Recombinant Adeno-Associated Virus. Journal of Virology, ...
  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...microinject into blastocysts to be implanted in pseudopregnant female mice. If you obtained the desired chimeric...microinjection, i-GONAD is performed directly on pregnant female mice using a small incision that exposes...
  6. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...then be exposed to a lysis buffer to release recombinant proteins directly onto the nitrocellulose for... 1 M NaCl in order to enhance folding of the recombinant proteins. Optimal salt concentration will vary...
  7. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...from multiple primer pairs, one can infer the predominant state of registers within a sample using a metric...states in a sample; instead, it will suggest the predominant state of registers in a sample based on a similarity...
  8. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...non-mutant line but no signal in the mutant line! Recombinant Strategies This approach is essentially the opposite...ready-to-use antibodies from Addgene Designing a recombinant validation experiment? Find plasmids here!  ...
  9. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...Zolotukhin S, Reier PJ, Mandel RJ, Muzyczka N (2004) Recombinant AAV Viral Vectors Pseudotyped with Viral Capsids... Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations from Viral...
  10. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...of dsRNA. In plants, CRISPR technologies are predominantly used to disrupt function at the level of DNA...Interference System in Physcomitrella patens Reveals a Dominant Role of Augmin in Phragmoplast Microtubule Generation...
  11. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...including indels. Therefore, they engineered a dominant-negative mutant of the protein MLH1, a component...prime editing systems by manipulating cellular determinants of editing outcomes. Cell, 184(22), 5635-5652...
  12. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...more reproducible/renewable reagents, such as recombinant or hybridoma-derived monoclonal antibodies. It...µg/mL (1/100). * = monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al., ...
  13. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from... plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids...
  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...phosphatase-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-...tool for use as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime...
  15. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...AAV9 in the liver. AAV9-X1.1 effectively and predominantly transduces neurons across brain regions, with...throughout the non-human primate brain. Nature Nanotechnology, 1–11. https://doi.org/10.1038/s41565-023-01419...
  16. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ...latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies."  Hot tip! This protocol can easily...
  17. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th day of CRISPR...
  18. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...giant paper mache Krampus performing routine lab maintenance tasks. Hot tip: make sure your PI/facilities ...
Showing: 161 - 180 of 326 results