We narrowed to 259 results for: nif

1. Plasmids 101: Screening Strategies Used in Plasmid Cloning

   Type

   Blog Post

   Published

   Sept. 15, 2020, 1:15 p.m.

   ... Jennifer Tsang...

2. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ...result, how most of these “variants of unknown significance” (sometimes called “VUS”) function is unknown...

3. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   Published

   March 30, 2017, 2:30 p.m.

   ...let’s explain what kind of assays can be used to sniff out your desired mutations. The assays you will ...

4. Antibodies 101: ChIP

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   Blog Post

   Published

   March 10, 2022, 3:25 p.m.

   ...Homogenization for a tissue sample can also need significant optimization for the sample type and size.) It...

5. Hot Plasmids - February 2022

   Type

   Blog Post

   Published

   Feb. 22, 2022, 2:15 p.m.

   ...is highly specific in mammalian cells without significant off-target effects, yet small enough to be packaged...

6. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   Published

   Feb. 8, 2022, 2:15 p.m.

   ...you're trapped in a pattern of small errors that significantly impacts your experiments. What do you do when...

7. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   Published

   Jan. 25, 2024, 2:15 p.m.

   ...solve the problem: CasPEDIA, a new resource from Jennifer Doudna’s lab at the Innovative Genomics Institute...

8. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ... technologies have idiosyncrasies presents a significant barrier for those wishing to use it as a tool...

9. Viral Vectors 101: The AAV Retrograde Serotype

   Type

   Blog Post

   Published

   April 27, 2023, 1:15 p.m.

   ...originally written by Leila Haery in 2017 and significantly updated in 2023 by Jason Nasse.  References...

10. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    Published

    Jan. 28, 2020, 2:35 p.m.

    ...enzymes implies that even a minute quantity can significantly reduce the operational time for a given process...

11. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...activity relative to Cas9. Since then, the Joung and Jennifer Doudna Labs used targeted mutagenesis to generate...

12. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    Published

    Dec. 6, 2018, 1:34 p.m.

    ...created an improved RNAi vector that leads to significantly stronger phenotypes than standard RNAi protocols...

13. 3 Challenges in Plant Synthetic Biology

    Type

    Blog Post

    Published

    July 22, 2014, 5:46 p.m.

    ... limited understanding of plant biology is a significant technical obstacle to plant synthetic biology...

14. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    Published

    Feb. 28, 2019, 1:34 p.m.

    ...learned, and to Sonali Roy, Magdalena Julkowska, and Jennifer Tsang for editing. Additional resources on the...

15. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    Published

    Sept. 4, 2018, 12:53 p.m.

    ... Jennifer Tsang...

16. RaPID Detection of RNA-protein Interactions

    Type

    Blog Post

    Published

    Aug. 16, 2018, 12:40 p.m.

    ...datasets. Applications of RaPID RaPID sounds like a nifty technique, but what kinds of questions can it help...

17. RANbodies: Reporter Nanobody Fusions

    Type

    Blog Post

    Published

    April 10, 2018, 12:56 p.m.

    ...antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially available antibodies...

18. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great gene therapy delivery mechanism...

19. Human Germline Editing Using CRISPR

    Type

    Blog Post

    Published

    Aug. 10, 2017, 2:19 p.m.

    ...biologists and non-biologists alike. Given the public significance of this research, we have the obligation to ...

20. Antibodies 101: Secondary Antibodies

    Type

    Blog Post

    Published

    Dec. 21, 2021, 2:15 p.m.

    ...which means that indirect methods are often significantly longer than direct ones.  Even though the secondary...