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Showing: 161 - 180 of 694 results

  1. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Last updated Sept 1, 2020. This post was contributed by David Wyatt and Dale Ramsden, UNC at Chapel Hill...Christina Mork contributed to the update of this post. Thank you to our guest bloggers!   David Wyatt...doi.org/10.1128/mbio.02364-19 More CRISPR Blog Posts from Addgene: CRISPR 101: Homology Directed Repair...

  2. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...be included in the next What’s New in CRISPR blog post, please let us know! Additional resources on the...Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org...

  4. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    Published
    Feb. 25, 2021, 2:15 p.m.
    ...and a positive perspective on the transition to a post-academic career. This training was “included” with...Addgene's science career resources Browse all blog posts related to science careers Download the Science ... it’s largely a matter of luck if a student or postdoc ends up in a lab where they can learn the plus ...development as core components of predoctoral and postdoctoral training. A grant from the National Institute...

  5. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    Published
    June 17, 2015, 9:04 p.m.
    ...other blog posts on sharing science: 10 Ways to Share Your Science More Scientific sharing posts! ...focused lectures, interactive panels, and a student poster session. All DayCon lecture content is open source...

  6. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...Modular assembly of designer PUF proteins for specific post-transcriptional regulation of endogenous RNA. J ...Addgene's Synthetic Biology Overview More Synbio Blog Posts ...

  7. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...This post was contributed by guest blogger Derek Simon. Optogenetics is spreading through the neuroscience...The Rockefeller University. In this and upcoming posts in this optogenetics primer series, I’m happy to...result in a particular neurotransmitter release or post-synaptic current. Fortunately, if your lab focuses...expression of your opsin, but keep you eyes open for posts about the next steps in the coming weeks. Expression...material science part of optogenetics. In next week’s post we’ll delve into some of the important materials...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

  8. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...microscopy, University of Amsterdam. The previous post in this series described a practical approach to...selecting a bright fluorescent protein. In the second post of this series, we will discuss how to select a ...

  9. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at the ... from the Allen Institute for Cell Science. This post was contributed by the gene editing team at the ...Non-Homologous End Joining Check Out Our Fluorescent Protein Posts Resources on Addgene.org Browse CRISPR Tagging...

  10. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...advantages that will be discussed in detail in a separate post. Focusing on the ccdB aspect, Gateway takes advantage...selecting for recombinant plasmids. Check out our blog post on Common Lab E. coli strains for more strain info...Golden Gate Cloning Read all of our Plasmid Cloning Posts Resources on Addgene.org Choose a Molecular Cloning...

  11. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    Published
    May 11, 2021, 1:15 p.m.
    ...This post was contributed by Adriana Galvan, an associate professor at Emory University School of Medicine...fluorescent illumination in in vivo, ex vivo, or post-mortem experiments. Fluorescent proteins can also...on the Addgene blog Browse Fluorescent 101 blog posts Read about chemogenetics vs. optogenetics Find more...

  12. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...Addgene blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn more about Lambda Red recombineering...This post was contributed by Jacob Lazarus, a postdoctoral researcher at Harvard. There’s an astounding...where he is now an attending physician. He performs post-doctoral research in the lab of Matthew Waldor at...

  13. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...within transmembrane proteins, such as receptors, or post-translational modifications on those proteins, like...decrease in signal strength in a flow plot. d = days post treatment. Created using biorender.com. Cell...Conclusion Congratulations! You made it through this blog post and hopefully learned a few new methods of target...briefly walk through the topics we covered in this post. Besides direct labeling, indirect labeling can ...

  14. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...like experiments (and their controls!). This blog post will discuss the ins and outs of controls for biological...approaches discussed in the Antibodies 101: Validation post. The primary antibody control needs only to be run...flow cytometry experiments. We hope that this blog post will be a useful starting point for you when designing...antibody-based experiments, check out these blog posts.       References and resources References Burry...

  15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...sometimes inte-great-ion isn’t so great. In this post, we’ll cover how standard lentiviral vectors work...proteins packaged during production (read our blog post on the retroviral life cycle for a more detailed...titration of vector DNA in samples harvested 24 hours post-transduction (Wanisch and Yáñez-Muñoz, 2009).  ...insert themselves into every conversation! This blog post was written by Addgenie Rebecca Hutcheson.   References...

  16. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...technologies are still being developed today. In this post, I will highlight the evolution of the  Brainbow... utility of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer...View the overview schematic at the bottom of this post for a basic synopsis of the differences amongst ...due to the addition of a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), commonly...

  17. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... blog post covering E. coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin... This post was updated on Nov 14, 2017. You've worked hard designing your plasmid – you carefully selected...Bacterial Expression Systems. Check out our companion post describing protein expression strains, where we ...

  18. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...viral service page and browse our viral vector blog posts for many more tips and ideas on how to use these...Expression in Mammals Subscribe to Viral Vector Blog Posts Additional Resources on Addgene.org Check out ...

  19. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ... find themselves faced with a roadblock. In this post, we will provide an overview of some of the common... tips on lentiviral titering please see our blog post. Between choosing the best lentiviral system, producing... to check out the lentiviral protocols and blog posts on Addgene’s website for additional resources. ...

  20. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...protocols for different experiments.  In this blog post let's take a look at some of the other open resources...   3’UTRome The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans. To study...Cas9 in C. elegans Browse our Plasmids 101 blog posts Resources on Addgene.org Visit our worm plasmids...
Showing: 161 - 180 of 694 results