We narrowed to 220 results for: sms
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TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....protein tagging. In two studies investigating mechanisms of endoplasmic reticulum localization, Munro ...
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10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....should keep in mind that UV light only kills microorganisms that it strikes directly. UV light exposure... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....Although FPs have enabled discovery of many cellular mechanisms, there are some limitations to working with FPs... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....double strand break (DSB) stimulates DNA repair mechanisms that can be exploited to either inactivate a ... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m....cancerous cells Luckily, there are several safety mechanisms in place to protect us from rogue cells when ... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m.... success of CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of its delivery methods... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated, MMEJ represents another... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....specimens and for in vivo imaging of transparent organisms. Advances in tissue clearing Similar to light ... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....25819219. Wang W, Roberts CJ. Protein aggregation - Mechanisms, detection, and control. Int J Pharm. 2018 Oct... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog PostPublishedAug. 27, 2020, 1:15 p.m.... such resources in less genetically tractable organisms lags behind. BiFC can also be used to screen for... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m....Resistance Stability Time Saving? Works for multiple organisms? Compatible with most bacteria? No satellite colonies... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....genomic sequence from one another) — by various mechanisms, only one of the two strands’ DNA counterparts... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation. The... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....chance of an opportunistic infection like for organisms like C. difficile. In 2014, Citorik et al. and... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans... -
A Career in Grants: How To Become a Grant Professional
TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m.... is rewarding and challenging! Jo Miller, GPC, CSMS is the founder and principal of J. Miller & Associates... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....bacteria and eukaryotes. But be careful — antibiotic mechanisms vary! Aminoglycoside antibiotics also block protein... -
CRISPR 101: Any Base Transversion Editors
TypeBlog PostPublishedApril 1, 2025, 1:15 p.m....concept for further development. Figure 2: Mechanisms of deaminase-based adenine transversion editors... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....of its biology, pathogenicity, and resistance mechanisms. Figure 4. Overview of the dual-plasmid...