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We narrowed to 243 results for: virus

Showing: 161 - 180 of 243 results

  1. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...simply fused the wild-type Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase to the C-terminus ...was made possible through their use of engineered virus-like particles. However, there’s still a long way...

  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...library to screen for genes involved in West Nile Virus (WNV) induced cell death. The Wu lab library consists.... PubMed PMID: 26837755   SEPTEMBER 2016 Zika virus plasmids Now avaliable through Addgene The Vaithi... Addgene’s repository. The genes, from the Zika virus Asian genotype PRVABC59, were obtained from the ...using direct transfection precluding the need for virus. Furthermore, dCAS9-VPR expression is under the ...First, GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors...use the library, the authors packaged it into lentivirus and used the resulting lentiviral library to ...dCas9-VPR limits one’s ability to package it into lentivirus and later create stable cell lines expressing...

  3. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...ready-to-use AAV preparations are labeled with the green virus particle on our biosensors page. Whether you’re...

  4. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...histidine-tagged protein expressed by recombinant vaccinia virus. Proc Natl Acad Sci U S A. 1991;88(20):8972-6. Moks...

  5. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...binds its own mRNA to facilitate its packaging into virus-like particles. Feng Zhang’s lab adapted the native...

  7. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ... the virus.  Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from... options for Cpf1 multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA... multiplex system from a lentiviral vector.  Lentiviruses carry a (+) strand RNA copy of the DNA sequence...

  11. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...helper plasmids were developed to replace helper viruses in AAV production systems. We’re just skimming ...plasmids” or “Col factors.” Virulence plasmids: microbial infection Virulence plasmids encode genes that...outbreaks of food poisoning, the difference is virulence plasmids! This category includes genes that help...instance, vectors derived from the Agrobacterium Ti virulence plasmid are now used routinely in biotechnology...

  12. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ... of insect cell expression systems include that virus production can be quite time consuming and that ...expression systems; baculovirus infected and non-lytic insect cells. Baculovirus expression systems are.... coli or yeast cells. The only problem with baculovirus systems is that the infected host cell is eventually...

  13. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...Figure 3: BaF3 cells were transduced with H2B-FT virus and grown in varying concentrations of IL-3. Cells...

  15. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...including cells, drugs, DNA, viral vectors, and viruses. All researchers must go through the proper basic...

  16. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...lab have developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive ...

  18. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ... consider including more background on how your virus of interest causes cancer, as well as therapeutic.... She studies the interaction between Human Papillomavirus and the innate immune system, and is interested...

  19. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...Gallagher, T. L., & Gao, G. (2024). Adeno-associated virus as a delivery vector for gene therapy of human diseases... while both AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost...made at high titers equal to those achieved with lentivirus but offer an improved safety profile, customization...expression in many mouse tissues. Safety AAV and lentivirus have different safety profiles that often affect...experiments. AAV can be safely handled at BSL-1, while lentivirus requires a BSL-2 setup. This requires a less ...unmatched by other viral vector delivery methods. Lentivirus has limited packaging options, with most lentiviral...the CRISPR components — are expressed. Unlike lentivirus, cargo delivered by AAV does not integrate into...
Showing: 161 - 180 of 243 results