We narrowed to 22 results for: 974
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TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m....mediate optogenetic inhibition. Nat Neurosci 25, 967–974 (2022). https://doi.org/10.1038/s41593-022-01094-...
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Antibodies 101: Immunoprecipitation
TypeBlog PostPublishedDec. 7, 2021, 2:15 p.m.... novel IgG-binding reagent. J Immunol 133(2):969-974. Hjelm H, Hjelm K, Sjöquist J (1972) Protein a from... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....transmitted by sand flies." Science 321.5891 (2008): 970-974. PubMed PMID: 18703742. PubMed Central PMCID: PMC2606057... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....their roadmap includes RNA → DNA (Cooper & Temin, 1974), as one of its first steps! However, retroviral...PMCID: PMC1170145 Cooper, G. M., & Temin, H. M. (1974). Infectious rous sarcoma virus and reticuloendotheliosis...1132–1141. https://doi.org/10.1128/JVI.14.5.1132-1141.1974. PubMed PMID: 4372391. PubMed Central PMCID: PMC355630... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog PostPublishedMarch 26, 2019, 1:40 p.m....121538 AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) 119741 AAV5 AAV SYN flex PSAM4 GlyR IRES EGFP Chimeric...Chimeric channels for neuronal manipulation 119742 AAV5 AAV SYN PSAM4 GlyR IRES EGFP Chimeric channels... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....stabilize the RNA molecules (Allewell and Sarma, 1974). Additionally, ThermoFisher provides a protocol... References Allewell NM, Sama A (1974) The effect of ammonium sulfate on the activity ... -
27 Hot Plasmids from 2016
TypeBlog PostPublishedDec. 22, 2016, 3:03 p.m....2005). In this kit, Lambda phosphatase (Plasmid 79748) enhances bacterial expression of Serine/Threonine...Threonine kinases while YopH (residues 164-468, Plasmid 79749) enhances expression of Tyrosine kinases. All sequences... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m.... al., Nucleic Acids Research 2018 PubMed: PMID 29746667. ... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog PostPublishedJune 20, 2017, 2:30 p.m....Methods Clin Dev. 2014 Aug 13;1:14034. PubMed PMID: 26015974. PubMed Central PMCID: PMC4420252 Additional Resources... -
New and Upcoming Viral Vectors - June 2019
TypeBlog PostPublishedJune 18, 2019, 1:15 p.m....New chemogenetics AAV Plasmid Serotype Name 119741 AAV5 AAV SYN flex PSAM4 GlyR IRES EGFP Recombinase... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....Press, pp 331–338 https://doi.org/10.1007/978-1-59745-181-9_23 Additional resources on the Addgene blog... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog PostPublishedMay 18, 2021, 1:15 p.m....al., bioRxiv 2021. https://doi.org/10.1101/2021.01.30.428974 The CRISPR corner By Jennifer Tsang Here... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog PostPublishedOct. 11, 2018, 12:30 p.m....." Nucleic acids research (2018). PubMed PMID: 29741723. PubMed Central PMCID: PMC6061786. Additional ... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m.... PubMed PMID: 27465542. PubMed Central PMCID: PMC4974478. Natsume, Toyoaki, et al. “Rapid Protein Depletion... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....Nucleic Acids Res. 2010;38(19):e184. Pubmed PMID: 20719741. Pubmed Central PMCID: PMC2965261. 8. Obrdlik,... -
Getting the Most from Your Lentiviral Transduction
TypeBlog PostPublishedApril 7, 2016, 2:30 p.m....Biotechniques 35.4 (2003). 812-4. Pubmed PMID: 14579747. 3. Drexler, H.G., Uphoff, C.C. “Mycoplasma contamination... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....genetics of Caenorhabditis elegans." Genetics 77.1 (1974): 71-94. PubMed Central PMCID: PMC1213120. Fire,... -
Plasmids 101: Dimers and Multimers
TypeBlog PostPublishedJan. 10, 2023, 2:15 p.m....707-16. doi: 10.1016/0092-8674(76)90134-3. PMID: 797459. Berg CM, Liu L, Coon M, Strausbaugh LD, Gray P... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m.... PubMed PMID: 27465542. PubMed Central PMCID: PMC4974478. 3. Schaub, F.X., et al. (2015) Fluorophore-NanoLuc... -
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog PostPublishedSept. 29, 2016, 2:30 p.m....Viruses." Chemical Reviews (2016). PubMed PMID: 27319741. 3. Simpson, David J., Jessica C. Sacher, and ...