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Showing: 1 - 9 of 9 results

  1. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...the Innovative Genomics Institute. What is CasPEDIA? CasPEDIA is an online, searchable resource that provides...a quick overview of each CasPEDIA feature. Activity features: CasID CasPEDIA will display both the cis...plasmids, Addgene and CasPEDIA have got you covered!     Fig. 1 – CasPEDIA Cas ID display information...for a Cas entry (SpyCas9a) from CasPEDIA.   How can I use it? CasPEDIA operates like many search engines...and embrace a new resource to solve the problem: CasPEDIA, a new resource from Jennifer Doudna’s lab at ...established uses, and intended applications. Within CasPEDIA, there is a classification system, CasID, to help...facilitate direct comparison of different enzymes. CasPEDIA is meant to be a constantly updating database ...

  2. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...activate a cascade of caspases, protease enzymes that cleave proteins. Executioner caspases are activated last... executioner caspase reporters. In these reporters, the FRET pair is linked by a caspase cleavage site...one of the linkers is a consensus caspase-3 (an executioner caspase) cleavage site (DEVD). Upon cleavage...used genetically encoded fluorescent executioner caspase reporters. Several apoptosis reporters have been...site. Cutting the linker by a caspase results in a change in fluorescence emission. FRET and fluorescence...Studying apoptosis in vivo using the FlipGFP-based caspase reporter The Shu Lab successfully used FlipGFP ...staurosporine, which induces apoptosis by activating caspase-3, and detected fluorescence within 2 to 5 hours...

  3. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    Published
    June 27, 2018, 1:16 p.m.
    ...specific genomic locus: Caspex  Article contributed by Susanna Bachle  Listen to the Caspex podcast segment ... proteins with biotin as an affinity tag. This ‘Caspex’ construct is guided by a pre-defined sgRNA sequence...at your locus of choice. Find the Tet-inducible caspex plasmid here. Myers SA et al. Nature Methods. ...

  4. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...(Mouse IgG2a) Goat anti-mouse IgG2a CASPR anti-CASPR mAb K65/35 (Mouse IgG1) Goat anti-mouse...Neocortex labeled with anti-pan-Nav (magenta), anti-CASPR (green), and anti-Kv2.1 (cyan). (B) Cerebellum labeled...

  5. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ... for a knock-out in your experiments? AAV-flex-taCasp3-TEVp from Nirao Shah's and Jim Well's lab induces...use viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1...

  7. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ... is not suitable for use in apoptotic cells as Caspase3/7 both cleave after the DVPD sequence. Check out...

  8. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...over to our CRISPR Plasmids and Resources page.  CasPhi is 70 kDa Cas protein identified exclusively in...

  9. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ... encyclopedia of Class 2 CRISPR systems called CasPEDIA, with information about enzyme activity, experimental...
Showing: 1 - 9 of 9 results