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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 29 results for: EXOG

Showing: 1 - 20 of 29 results

  1. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...the control happening? Is the regulation of an exogenously introduced gene or over the native locus? Let... systems work by tagging the endogenously or exogenously expressed protein with the relevant tag and then... These systems can be easily implemented for exogenously introduced proteins, as many of them just need...

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...popular for the modulation of endogenously and exogenously expressed proteins. Here we will review what ...certain conditions (typically the addition of an exogenous small molecule). Degron tags can range in size...tags can be used to manipulate endogenously and exogenously expressed proteins. Thirdly, degrons and their...

  3. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ... fall into two categories: those that rely on exogenous enzymes and those that use endogenous homologous...recombination. Modifying bacterial genomes using exogenous enzymes: Lambda red and CRISPR Developed over ... these systems are powerful, but they rely on exogenous enzymes, require multiple transformation and plasmid...

  4. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...thi1 Mutation in thiamine metabolism Requires exogenous thiamine for growth ara disruption of arabinose...β-isopropyl malate dehydrogenase inactivated Requires exogenous leucine source for growth proAB mutation in ...in proline biosynthesis pathway Requires exogenous proline source for growth rpsL Mutation in subunit...

  5. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...result, gene silencing can be easily induced by exogenous application of long or short dsRNA. However, it...unmatched—unlike CRISPR experiments that require exogenous protein, successful induction of RNAi only requires...microRNAs found in the genome, whereas shRNAs are exogenously derived. Importantly, instead of the assortment...

  6. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...thermophilus to predatory phages to test if the exogenous DNA would be incorporated into the bacterial genome..., which guide a Cas nuclease to the specified exogenous genetic material next to the species-specific ...

  7. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ... 2019 Arnaud Gautier HaloTag TagBiFC allows exogenous labeling for single-molecule imaging TagBiFC technique...provides maximum flexibility in color labeling with exogenous fluorophores, which can even be bright enough ...

  12. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...Membrane receptors and ion channels are often exogenously expressed and studied in HEKs due to the cell...

  14. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... it may be possible to direct insertion of an exogenous DNA fragment at a Cas9 targeted break (or pair...

  17. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...with toxicity to many organisms. Fig. 1: Exogenous sources of dsRNA Cellular sources of dsRNA...

  19. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...vivo or in vitro and a small molecule is added exogenously to measure a phenotypic response in the form ...

  20. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ... wanted to distinguish between endogenous and exogenous versions of the same target. In the 1980s, Munro...
Showing: 1 - 20 of 29 results