... cloning of firefly luciferase was published in 1985, several studies utilized luciferase as a genetic... Biological sources of luciferase Although luciferase isolated from the firefly beetle (Photinus pyralis... plasmids expressing luciferase Luciferase reporter assay The typical luciferase-containing plasmid is...second most commonly used luciferase. Other organisms from which luciferases have been cloned from include... North American firefly(Photinus pyralis) 560 61 Luciferin ATP No Japanese firefly(Luciola cruciate...cruciate/lateralis)Italian firefly(Luciola italica)Southern Russian firefly(Luciola mingrelica) 562 64 Luciferin...containing luciferase and the gene of interest permits translation of functional luciferase, until miRNA...

...fusion proteins from any ORF available as a standard entry clone. ORFs from an ORFeome Gateway library or any...Dreyfuss lab has developed rapid-response luciferase (firefly P. pyralis) reporter plasmids for use in ...alternative to the widely used luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids... issues with fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash...insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation of its substrate, luciferin...Santalucía, you can now take any open reading frame (ORF) and with a simple recombination reaction, generate... any PCR-amplified ORF cloned into pDONR221 can be expressed either without a tag or as multiple fusions...