28 Hot Plasmid Technologies from 2015
Type
Blog Post
Published
Dec. 23, 2015, 3:30 p.m.
...fusion proteins from any ORF available as a standard entry clone. ORFs from an ORFeome Gateway library or any...Dreyfuss lab has developed rapid-response luciferase (firefly P. pyralis) reporter plasmids for use in ...alternative to the widely used luciferase reporter gene plasmid pGL3
pGL3 luciferase reporter gene plasmids... issues with fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash...insensitivity to other ions
In BRET, A mutant Renilla luciferase, catalyzes the oxidation of its substrate, luciferin...Santalucía, you can now take any open reading frame (ORF) and with a simple recombination reaction, generate... any PCR-amplified ORF cloned into pDONR221 can be expressed either without a tag or as multiple fusions...