We narrowed to 3 results for: GCT

1. Plasmid Cloning by PCR

   Type

   Blog Post

   Published

   March 29, 2016, 2:30 p.m.

   .... This gives us a sequence of 5'-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3' (30bp with 18bp of homology to...chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...

2. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

   Type

   Blog Post

   Published

   June 30, 2020, 1:15 p.m.

   ...recently attended virtual conferences (TAGC and ASGCT, respectively) and Addgenie Angela Abitua hosted...

3. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

   Type

   Blog Post

   Published

   July 8, 2025, 1:15 p.m.

   ... 3’-TACT flanking promoter parts, 5’-AATG and 3’-GCTT flanking CDS parts, etc). These overhangs are known...