...fluorescent protein topics page Get the basics on GFP Find microbiology blog posts Resources on Addgene.org...variant library is monitored by fusing the variants to eGFP followed by an internal ribosomal entry site and...a variant has wild-type levels of abundance, the eGFP will be stable and fluoresce. But if the variant...unstable, it will be degraded, leading to reduced eGFP fluorescence. Cells expressing the library are then...fluorescence-activated cell sorting into four bins based on their eGFP:mCherry ratios. Genomic DNA from each bin are then extracted...

...library comes either with or without mCherry and GFP fluorescent controls. Alternatively, you can order...

...be another method to assess transfection success. GFP is often used as a reporter and we will be covering...

...selection cassette. They could also switch from FLAG to GFP or a different epitope. Addgene: Do you have any ...

...cells in vivo, the non-immunogenic violet-excited GFP variant Vex. To understand the impact of this gene...

...Expression with Cre-lox Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids...

...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free...

... for a researcher to come into contact with than GFP. Similarly, if the viral vector carries an shRNA ...

...line, Hep3B. From left to right: Mitotracker Red, GFP for the biosensor, Hoechst, and overlay).   ...(DKFZ) who has developed new redox sensitive GFP2 (roGFP2)-based biosensors for live cells. His work focuses..., Pedre Pérez’s biosensor fuses MPST to roGFP2. Because roGFP2 contains two cysteines near the chromophore...the MPST hydrosulfide can be transferred to the roGFP2 cysteine thiols and change its fluorescence properties...

...Addgene has several great options: pLV hUbC-dCas9-T2A-GFP for removable dCas9 expression and pLM-CMV-R-Cre....

... enriched relative to controls using Split-BioID-GFP and BioID run on six unrelated proteins. One caveat...

...select both over- and under-expressing clones. If GFP-encoding cDNA is co-transfected at a favorable ratio...

..., Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess Cas9-PAGE editing with viral...

...contrast, the widely used green fluorescent protein (GFP) gives a bright green fluorescence when exposed to...eforRed, asPink, spisPink, scOrange, fwYellow, amilGFP, amajLime, cjBlue, meffBlue, aeBlue, amilCP, tsPurple...

...Mammalian Cells Learn about the Dangers of Using GFP for Protein Localization Resources on Addgene.org...

...check out our article: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection. Cellular...

...which must be stabilized by monomeric superfolded GFP and averages only ~50% RNA knockdown. In initial ...

...Lindsey M. Costantini, and Erik L. Snapp. "Superfolder GFP is fluorescent in oxidizing environments when targeted...

...in this case a reporter gene (e.g. luciferase or GFP), regardless of whether it interacts with a transcriptional...

...SYFP2 Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-...