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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6 results for: KAS III;

Showing: 1 - 6 of 6 results

  1. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...plasmid in the host bacterium that expresses gene III. Gene III is under the control of a T7 RNA polymerase ...bacteriophage, which rely on the expression of gene III to create infectious particles. A plasmid-based genetic...circuit in the bacteria links the expression of gene III to base editor activity and editing speed and consists...active variant of the base editor, a functional gene III product is produced and can infect bacterial cells...circuit in the bacteria links the expression of gene III to base editor activity and editing speed. Created...catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying enzyme, in this case ...

  2. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ... to have derived from the more complex type III-A and III-B systems. Though it was composed of many protein...ancestors. They classified the system as Class I, Type III-E, and then used this information to identify a large...Cian Schmitt-Ulms, Rohan N. Krajeski, Justin Lim, Lukas Villiger, Wenyuan Zhou, Kaiyi Jiang, Nathaniel Roberts... Roberts, Liyang Zhang, Christopher A. Vakulskas, John A. Walker II, Anastasia P. Kadina, Adrianna E. ...

  3. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...single guide RNA (protospacer+scaffold) in a pol-III (OsU3) promoter driven expression system. We use ...editors by tethering cytosine deaminase with Cas9 nickase (nCas9). nCas9 creates a nick in the DNA by cutting...of the base editors mentioned above are SpCas9 nickase-based and depend on the presence of an ‘NGG’ PAM...PAM sequences other than NGG (Table-1). Type Nickase Cas9 (D10A) Deaminase PAM requirements Reference...

  4. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...reasons to multiplex gRNAs, some being: Using dual nickases to generate a knockout or edit. This can help ...variants are available: wt humanized Cas9, D10A nickase mutant (Cas9n), dCas9 (transcriptional repression...Comparison of Multiplex Strategies including Standard PolIII-gRNA cassette, Csy4-cleavable cassette, and PTG...

  5. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...RNA structure that is recognized and cleaved by RNaseIII resulting in cell survival. Plasmid addiction... 9.4 (2009): 600-609. PubMed PMID: 19416369. Kulakauskas, Saulius, Arvydas Lubys, and S. Dusko Ehrlich...

  6. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...targeting sequence of cytochrome c oxidase subunit VIII, so that they can be imported to the mitochondrial...TALEN assembly and activity? The laboratory of Takashi Yamamoto has created a complete TALEN assembly ...
Showing: 1 - 6 of 6 results