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Addgene

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We narrowed to 7 results for: MK

Showing: 1 - 7 of 7 results

  1. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... hsdR(rk-, mk+) Unmethylated DNA not degraded, cell still can methylate DNA   hsdS(rk-,mk-) Unmethylated... nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ– HB101 Streptomycin Hybrid of E. coli K12 ... e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+) JM110  Streptomycin  For storing plasmids that... e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+)  MC1061 Streptomycin Parent of DH10B/TOP10 and...glnV44 F'[ ::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(rK- mK+)  XL10 Gold Tetracycline and Chloramphenicol High...

  2. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...propensity to aggregate. These monomeric FP timers (mK-GO from Tsuboi et al. and the FT series from Subach...mCherry, respectively. While Tsuboi et al developed mK-GO somewhat serendipitously while attempting to enhance... of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT series matures...membrane by fusing various vesicle cargoes to FP timer mk-GO (Tsuboi et al.) Monitor gene expression in the...

  3. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was designed to work in fungi... interchangeable across organisms. When using the MK system, many of the parts can be used in a variety...and money for labs with diverse applications. The MK system was used successfully for protein localization...

  5. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    .../preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla I (...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...main FTs are called Slow-FT, Medium-FT, Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named...

  7. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...Maucksch C, Bohla A, Hoffmann F, Schleef M, Aneja MK, Elfinger M, Hartl D, Rudolph C. Transgene expression...
Showing: 1 - 7 of 7 results