Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 10 results for: MK

Showing: 1 - 10 of 10 results

  1. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ... hsdR(rk-, mk+) Unmethylated DNA not degraded, cell still can methylate DNA   hsdS(rk-,mk-) Unmethylated... nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ– HB101 Streptomycin Hybrid of E. coli K12 ... e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+) JM110  Streptomycin  For storing plasmids that... e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+)  MC1061 Streptomycin Parent of DH10B/TOP10 and...glnV44 F'[ ::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(rK- mK+)  XL10 Gold Tetracycline and Chloramphenicol High...

  2. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...propensity to aggregate. These monomeric FP timers (mK-GO from Tsuboi et al. and the FT series from Subach...mCherry, respectively. While Tsuboi et al developed mK-GO somewhat serendipitously while attempting to enhance... of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT series matures...membrane by fusing various vesicle cargoes to FP timer mk-GO (Tsuboi et al.) Monitor gene expression in the...

  3. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was designed to work in fungi... interchangeable across organisms. When using the MK system, many of the parts can be used in a variety...and money for labs with diverse applications. The MK system was used successfully for protein localization...

  4. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ... M NaCl/PBS-MK buffer 25% iodixanol step: mix 5 mL of 60% iodixanol and 7 mL of 1X PBS-MK buffer and 30...blunt edge needles, Hamilton 1X PBS pH 7.4 1X PBS-MK buffer 100X Pluronic-F68 NaCl MgCl 2 KCl Centrifugal...units (MWCO 100 kDa) Reagent Preparation 1 M NaCl/PBS-MK buffer Dissolve 5.84 g of NaCl, 26.3 mg of MgCl 2...through a 0.22 μm filter and store at 4 °C. 1X PBS-MK buffer Dissolve 26.3 mg of MgCl 2 , and 14.91 mg ... mix 6.7 mL of 60% iodixanol and 3.3 mL of 1X PBS-MK buffer 60% iodixanol step: mix 10 mL of 60% iodixanol...

  6. Summer SciComm: Preprints

    Type
    Blog Post
    .../preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla I (...

  9. Molecular Biology Reference

    Type
    Guide
    ...Phi80lacZDeltaM15 Delta(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA JM109 Addgene...Promega e14-(McrA-) recA1 endA1 gyrA96 thi-1 hsdR17(rK- mK+) supE44 relA1 Delta(lac- proAB) [F traDelta36 proAB...
Showing: 1 - 10 of 10 results