Plasmids 101: Common Lab E. coli Strains
Type
Blog Post
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hsdR(rk-, mk+)
Unmethylated DNA not degraded, cell still can methylate DNA
hsdS(rk-,mk-)
Unmethylated... nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ–
EPI300
Cloning large inserts and transformation... e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+)
JM110
Streptomycin
For storing plasmids that... e14- [F' traD36 proAB+ lacIq lacZΔM15] hsdR17(rK-mK+)
MC1061
Streptomycin
Parent of DH10B/TOP10 and...glnV44 F'[ ::Tn10 proAB+ lacIq Δ(lacZ)M15] hsdR17(rK- mK+)
XL10 Gold
Tetracycline and Chloramphenicol
High...