AAV Purification by Iodixanol Gradient Ultracentrifugation
Type
Protocol
... M NaCl/PBS-MK buffer 25% iodixanol step: mix 5 mL of 60% iodixanol and 7 mL of 1X PBS-MK buffer and 30...blunt edge needles, Hamilton 1X PBS pH 7.4 1X PBS-MK buffer 100X Pluronic-F68 NaCl MgCl 2 KCl Centrifugal...units (MWCO 100 kDa) Reagent Preparation 1 M NaCl/PBS-MK buffer Dissolve 5.84 g of NaCl, 26.3 mg of MgCl 2...through a 0.22 μm filter and store at 4 °C. 1X PBS-MK buffer Dissolve 26.3 mg of MgCl 2 , and 14.91 mg ... mix 6.7 mL of 60% iodixanol and 3.3 mL of 1X PBS-MK buffer 60% iodixanol step: mix 10 mL of 60% iodixanol...