Fluorescent Biosensors for Measuring Autophagic Flux
Type
Blog Post
Published
Jan. 22, 2019, 2:41 p.m.
...at Addgene!
mRFP-GFP-LC3 tandem fluorescent protein
This autophagy biosensor contains mRFP and GFP fused...commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as an autophagy bionsensor via...Rosella: GFP is quenched in acidic environments, while mRFP is relatively stable and fluorescences even at the...yellow puncta but a decrease in red puncta.
Find the mRFP-GFP-LC3 plasmid at Addgene!
GFP-LC3-RFP-LC3∆G: A...the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein, but following...