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Showing: 41 - 60 of 180 results

  1. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...which will feature a simplified user interface, expanded numbers of curated molecules, including 400 or...or so coregulators, and a greatly expanded set of links to datasets and reagents in the field. Additional...

  2. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...the data-driven approach was needed to massively expand complexity. The three tools developed, outlined...specific overhang sequences, allowing it to be used to expand already-existing sets. Note that this tool relies...

  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...2015). Recently the Ward lab created a tool-kit to expand upon these original tools that allows for both ... New INTRSECT viral preps available!  We are expanding our inventory of ready to use viral preps from...

  4. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ... Yongsub Kim’s lab engineered prime editors to expand their PAM flexibility. Find the engineered prime...some of the new viral preps from recent months: Expanded serotypes are now available for Gordon Fishell...

  5. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...interests while also staying on top of the ever expanding body of biomedical literature! PubMed PubMed ...researchers, journals, and concepts to help you expand your search. Results in your feed are ranked based...

  7. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...AAV1! New INTRSECT viral preps available! We are expanding our inventory of ready to use viral preps from...imaging calcium dynamics in fine structures. We have expanded our recombinase-dependent virus selection to now...

  8. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...for lists of alternatives to BLAST: ExPASy - http://www.expasy.org/genomics/sequence_alignment EMBL-EBI...

  9. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...., Grames, M. S., & Klein, R. L. (2018). More expansive gene transfer to the rat CNS: AAV PHP.EB vector...with Addgene’s AAV Data Hub!Data Freedom: The Expansion of Data Sharing in Research Publications...

  10. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...then read on for your best options. There are an expanding number of Cas9s with looser PAM requirements compared...Cas9s can be found in “The PAM Requirement and Expanding CRISPR Beyond SpCas9.” Single base pair edits ...

  11. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...video to complement traditional print journals and expand our ability as researchers to reach new audiences...Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications Resources...

  12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...C2c2), has several unique properties that further expand the CRISPR toolbox. We'll cover how Cas13a was ...diversity to the CRISPR toolbox and will continue to expand the applications of CRISPR. Since this post was...

  13. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...through the development of tools that exploit and expand our current knowledge. The Abudayyeh-Gootenberg... Lately, they’ve been working on two tools that expand and exploit CRISPR, both of which we're featuring...

  14. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...alternatives to GFP is expected to grow with the ever-expanding genetic toolbox generated by the continuous effort...PubMed PMID: 28401765. 15. Takai, Akira, et al. "Expanded palette of Nano-lanterns for real-time multicolor...

  16. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...after your training. You must ensure that you are expanding your options and preparing for both academic and...conflicts PI has no idea who is dating whom Expanding your skillset Scientists are lifelong learners...

  17. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ... offers additional PAM options that can greatly expand gRNA options. Two newer technologies offer an alternative...exciting time for functional genomics, with an ever-expanding list of tools to probe gene function. The best...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...sequence homology (usually 30-40bp overlaps). To expand the capabilities of Gibson assembly and make a ...scientists use GMAP, there will be a continuous expansion of compatible backbones, promoters, and genes ...depositing your new plasmids with Addgene to help expand the collection! The Jacks lab has made several ...

  19. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...cle10ts) allele), increasing experimental options. Expanding and improving the CRISPR/Cas editing toolbox for...need to segregate away co-conversion markers. Expanding beyond Cas9 Since the development of CRISPR/Cas9...

  20. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering ...protein function and signaling networks continues to expand. The labs of Jesse Rinehart and Dieter Söll at ...
Showing: 41 - 60 of 180 results