...219–225 . https://doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova KS, Shmakov S, Koonin EV (2017..., Cress BF, Crits-Christoph A,  He C , Xu M, Zhou Z, Smock DC, Tang K, Owens TK, Krishnappa N, Sachdeva.../2020.07.17.209189v2 Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin EV, Zhang F ...Sun X, Wang Q, Xu J, Dong F, Yang S, Yang J, Zhang Z, Qian Y, Chen J, Zhang J, Liu Y, Tao R, Jiang Y, Yang...communities and into species not previously cultured or isolated (Rubin et al., 2020).  Figure 3: Multiplexed...

...j.cell.2014.09.039 Zhou H, Liu J, Zhou C, Gao N, Rao Z, Li H, Hu X, Li C, Yao X, Shen X, Sun Y, Wei Y, Liu...Housden BE, Perrimon N, Collins JJ, Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods...

...Resources References Radtke, A. J., Chu, C. J., Yaniv, Z., Yao, L., Marr, J., Beuschel, R. T., Ichise, H., ..., Gola, A., Thakur, N., Shih, R., Yao, L., Yaniv, Z. R., Beuschel, R. T., Kabat, J., Croteau, J., Davis...type, target species, target name, host organism, isotype, clone details, vendor, method, tissue preservation...with Addgene Antibodies 101: Choosing the Right Isotype...

...Villette, V., Bradley, J., Wang, Z., Jiang, D., Zhang, G., … Lin, M. Z. (2024). A fast and responsive voltage... Find more neuroscience antibodies here! Wu, Z., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry...neurodegenerative diseases (Alzheimer’s disease or Hoarding disorder), but not in a patient with non-neurodegenerative...

...MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung ...Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR (2018) Evolved Cas9 variants with broad PAM...bacterial species Many more Cas9 orthologs have been isolated, and our understanding of their PAMs is shown ...same species. Expanding the CRISPR toolbox The isolation of novel CRISPR proteins has and will continue...

...doi.org/10.2144/000112585   Ding T, Huang C, Liang Z, Ma X, Wang N, Huo Y-X (2020) Reversed paired-gRNA.... https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank C, Aneja MK, Rudolph C (2019) Segmented...recombination in samples. For a more detailed comparison of these cloning strains, browse the table of...preps. In some cases, we suggest gel extraction to isolate the full, intact plasmid. Please see our gel extraction...

...9-39 Gao Q, Xu W, Yan T, Fang X, Cao Q, Zhang Z, Ding Z, Wang Y, Wang X (2019) Rescue of a plant cytorhabdovirus....org/10.1104/pp.15.00636 Ma X, Zhang X, Liu H, Li Z (2020) Highly efficient DNA-free plant genome editing...often requiring several rounds of selection to isolate plants with the phenotype of interest. Genome engineering...

...20616. https://doi.org/10.1074/jbc.M101559200 Wang, Z., Zhu, T., Rehman, K. K., Bertera, S., Zhang, J., ...//doi.org/10.2337/diabetes.55.04.06.db05-0927 Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated.... https://doi.org/10.1016/j.ymthe.2006.05.009 Wu, Z., Miller, E., Agbandje-McKenna, M., & Samulski, R....-stock viral prep service.  AAV2 Despite being isolated second, AAV2 is the best studied and most commonly..., K. L., Ayasoufi, K., & Barry, M. A. (2019). Comparison of Gene Delivery to the Kidney by Adenovirus,...

...Gao, H., Bai, S., Li, C., Ding, R., Xue, N., Lei, Z., Chen, Y., Guan, Y., Siwko, S., … Li, D. (2023). ...., Miyabe, A., Araki, M., Hara, K. Y., Shimatani, Z., & Kondo, A. (2016). Targeted nucleotide editing ... 883–891. https://doi.org/10.1038/s41587-020-0453-z Thuronyi, B. W., Koblan, L. W., Levy, J. M., Yeh, ... G., Ma, X., Geng, H., Huang, W., Pang, X., Yang, Z., … Li, D. (2020). Dual base editor catalyzes both...favorably to other methods. In a head-to-head comparison with Cas9, Cas9 modified 9/12 known off-targets...

...target in the parental cell line, but not in the iso-genic knockout control. This can involve cell surface... Schwartz, J., Kolahdouzan, M., Chen, C. X., You, Z., Benaliouad, F., Gileadi, O., McBride, H. M., Durcan.../doi.org/10.1111/bph.15467 Virk, H. S., Rekas, M. Z., Biddle, M. S., Wright, A. K. A., Sousa, J., Weston...

...share updated versions of the kit.” Reference Abil Z, Denard CA, Zhao H.  Modular assembly of designer ... protein that binds an alternate sequence. By comparison, Abil explained, the specificity of other RNA...

...Paperwork. Vincent BJ, Scholes C, Staller MV, Wunderlich Z, Estrada J, Park J, Bragdon MD, Lopez Rivera F, Biette... for career planning, it shouldn’t be used in isolation. Without clear dialogue between trainee and PI...Strategies to Resolve Conflict With Difficult Supervisors Management for Scientists: Seeking Feedback Management...

...Banskota, S., Davis, J. R., Newby, G. A., Chen, P. Z., Palczewski, K., & Liu, D. R. (2024). Engineered ...Nuñez, J. K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., ...problematic for low-abundance targets or natively isolated complexes. To address these limitations, the Funabiki...Funabiki Lab developed a technique called Magnetic Isolation and Concentration (MagIC)-cryo-EM that combines...

...imaging of voltage fluctuations. Image credit: Liu Z, et al. Cell. 2022.  Find JEDI-2p viral preps here...13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a) antibody is currently recommended for use...here!   Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-...

...278–282. https://doi.org/10.1038/s41586-021-03676-z  More resources on the Addgene blog Introduction ...Arbabi-Ghahroudi, 2017)!      Figure 1: Comparison of a heavy-chain only antibody and single-domain...

...inactivation. Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, Sullender M, Ebert BL, Xavier RJ...Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root...testament to how far genome engineering has come. For comparison, in 2007 it took about 3 months to knockout a... Generating Stable Cell Lines with Lentivirus Isolating a Monoclonal Cell Population by Limiting Dilution...

... 219–225. https://doi.org/10.1038/s41586-019-1323-z Peters, J. E., Makarova, K. S., Shmakov, S., & Koonin...outcompete their brethren, and the researchers isolated these more efficient CASTs (Witte et al., 2025...

...1 (2020). https://doi.org/10.1186/s12929-019-0592-z  Resources Antibodies 101: Introduction to Antibodies...disease conditions, from cancer to autoimmune disorders. Antibodies administered as drugs are still immunogenic..., and can be used as a whole or can be used to isolate specific subsets like CD4+ or CD8+ T-cells or monocytes...

...PMID: 31752513. Zhou, Q., Tian, W., Liu, C., Lian, Z., Dong, X., & Wu, X. (2017). Deletion of the B-B’ ...fmed.2023.1106085. PMID: 36817775. Wilmott, P., Lisowski, L., Alexander, I. E., & Logan, G. J. (2019). ...

...Central PMCID: PMC4916159. 2. Jacobson, K., Rajfur, Z., Vitriol, E., & Hahn, K. (2008). Chromophore-assisted...Visit Our Fluorescent Protein Guide Page Check out MiniSOG2 and SuperNova, two other photosensitizers used...