... PMID: 23287718 Cui, Z., Tian, R., Huang, Z., Jin, Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo... Liu, Y., Gu, Z., Dickerson, K. E., Xie, S., Hon, G. C., Xuan, Z., Zhang, M. Q., Shao, Z., & Xu, J. (2017...Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee... 35236982 Bubeck, F., Hoffmann, M. D., Harteveld, Z., Aschenbrenner, S., Bietz, A., Waldhauer, M. C., ...H., Zhou, R., Lang, B., Meng, B., Weng, H., & Hu, Z. (2022). FrCas9 is a CRISPR/Cas9 system with high ... 24463574 Gao, S., Wang, Y., Qi, T., Wei, J., Hu, Z., Liu, J., Sun, S., Liu, H., & Wang, Y. (2023). Genome...Sun, N., Zeina, C. M., Gao, X., Rees, H. A., Lin, Z., & Liu, D. R. (2018). Evolved Cas9 variants with ...

...MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JR, Aryee MJ, Joung JK...PMID: 25307932 Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O,... Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, Joung JK. 2016. High-fidelity CRISPR-Cas9 nucleases...: 24463574 Fujita T, Fujii H. 2013. Efficient isolation of specific genomic regions and identification...iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification...

...Singh A, Kuksenko O, Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell ...collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones...purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that simplifies identification...identification of the transcription factor isoform in pooled screens. This collection and library can be...transcription factor open reading frames. Each isoform is uniquely barcoded with a 24 bp sequence, to ...

...219–225 . https://doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova KS, Shmakov S, Koonin EV (2017..., Cress BF, Crits-Christoph A,  He C , Xu M, Zhou Z, Smock DC, Tang K, Owens TK, Krishnappa N, Sachdeva.../2020.07.17.209189v2 Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin EV, Zhang F ...Sun X, Wang Q, Xu J, Dong F, Yang S, Yang J, Zhang Z, Qian Y, Chen J, Zhang J, Liu Y, Tao R, Jiang Y, Yang...communities and into species not previously cultured or isolated (Rubin et al., 2020).  Figure 3: Multiplexed...

... D, Huang C, Huff S, Hunker A, Johansen N, Juneau Z, Kalmbach B, Khem S, Kussick E, Kutsal R, Larsen R...Walker M, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, Ariza J, Dee N, Reding M, Ronellenfitch K...Goldy J, Cetin AH, Smith K, Way SW, Esposito L, Yao Z, Gradinaru V, Sunkin SM, Lein E, Levi BP, Ting JT,...CT Isocortex 230801 pAAV-AiE2575m-minBG-iCre(R297T)-BGHpA AiP20139 AiE2575m Cre Layer 6_CT Isocortex 214601... 6b Isocortex 220729 pAAV-AiE2586m-minBG-iCre(R297T)-BGHpA AiP2136 AiE2586m Cre Layer 6b Isocortex 230554... 6b Isocortex 220728 pAAV-AiE2587m-minBG-iCre(R297T)-BGHpA AiP2068 AiE2587m Cre Layer 6b Isocortex 163505...for cell populations in the following regions: Isocortex Hippocampal formation Cortical subplate Striatum...

...j.cell.2014.09.039 Zhou H, Liu J, Zhou C, Gao N, Rao Z, Li H, Hu X, Li C, Yao X, Shen X, Sun Y, Wei Y, Liu...Housden BE, Perrimon N, Collins JJ, Church G (2016) Comparison of Cas9 activators in multiple species. Nat Methods...

...Resources References Radtke, A. J., Chu, C. J., Yaniv, Z., Yao, L., Marr, J., Beuschel, R. T., Ichise, H., ..., Gola, A., Thakur, N., Shih, R., Yao, L., Yaniv, Z. R., Beuschel, R. T., Kabat, J., Croteau, J., Davis...type, target species, target name, host organism, isotype, clone details, vendor, method, tissue preservation...with Addgene Antibodies 101: Choosing the Right Isotype...

...Villette, V., Bradley, J., Wang, Z., Jiang, D., Zhang, G., … Lin, M. Z. (2024). A fast and responsive voltage... Find more neuroscience antibodies here! Wu, Z., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry...neurodegenerative diseases (Alzheimer’s disease or Hoarding disorder), but not in a patient with non-neurodegenerative...

...MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung ...Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR (2018) Evolved Cas9 variants with broad PAM...bacterial species Many more Cas9 orthologs have been isolated, and our understanding of their PAMs is shown ...same species. Expanding the CRISPR toolbox The isolation of novel CRISPR proteins has and will continue...

...doi.org/10.2144/000112585   Ding T, Huang C, Liang Z, Ma X, Wang N, Huo Y-X (2020) Reversed paired-gRNA.... https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank C, Aneja MK, Rudolph C (2019) Segmented...recombination in samples. For a more detailed comparison of these cloning strains, browse the table of...preps. In some cases, we suggest gel extraction to isolate the full, intact plasmid. Please see our gel extraction...

...9-39 Gao Q, Xu W, Yan T, Fang X, Cao Q, Zhang Z, Ding Z, Wang Y, Wang X (2019) Rescue of a plant cytorhabdovirus....org/10.1104/pp.15.00636 Ma X, Zhang X, Liu H, Li Z (2020) Highly efficient DNA-free plant genome editing...often requiring several rounds of selection to isolate plants with the phenotype of interest. Genome engineering...

...20616. https://doi.org/10.1074/jbc.M101559200 Wang, Z., Zhu, T., Rehman, K. K., Bertera, S., Zhang, J., ...//doi.org/10.2337/diabetes.55.04.06.db05-0927 Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated.... https://doi.org/10.1016/j.ymthe.2006.05.009 Wu, Z., Miller, E., Agbandje-McKenna, M., & Samulski, R....-stock viral prep service.  AAV2 Despite being isolated second, AAV2 is the best studied and most commonly..., K. L., Ayasoufi, K., & Barry, M. A. (2019). Comparison of Gene Delivery to the Kidney by Adenovirus,...

...-mEGFP AICSDP-63 mEGFP Alpha-actinin-2 Sarcomeric z-disks 124608 NPM1-mTagRFP-T AICSDP-69 mTagRFP-T Nucleophosmin...SLC25A17-mEGFP AICSDP-27 mEGFP Peroxisomal membrane protein PMP34 Peroxisomes 101785 TUBA1B-mTagRFP-T AICSDP...

...Gao, H., Bai, S., Li, C., Ding, R., Xue, N., Lei, Z., Chen, Y., Guan, Y., Siwko, S., … Li, D. (2023). ...., Miyabe, A., Araki, M., Hara, K. Y., Shimatani, Z., & Kondo, A. (2016). Targeted nucleotide editing ... 883–891. https://doi.org/10.1038/s41587-020-0453-z Thuronyi, B. W., Koblan, L. W., Levy, J. M., Yeh, ... G., Ma, X., Geng, H., Huang, W., Pang, X., Yang, Z., … Li, D. (2020). Dual base editor catalyzes both...favorably to other methods. In a head-to-head comparison with Cas9, Cas9 modified 9/12 known off-targets...

...target in the parental cell line, but not in the iso-genic knockout control. This can involve cell surface... Schwartz, J., Kolahdouzan, M., Chen, C. X., You, Z., Benaliouad, F., Gileadi, O., McBride, H. M., Durcan.../doi.org/10.1111/bph.15467 Virk, H. S., Rekas, M. Z., Biddle, M. S., Wright, A. K. A., Sousa, J., Weston...

...share updated versions of the kit.” Reference Abil Z, Denard CA, Zhao H.  Modular assembly of designer ... protein that binds an alternate sequence. By comparison, Abil explained, the specificity of other RNA...

...Paperwork. Vincent BJ, Scholes C, Staller MV, Wunderlich Z, Estrada J, Park J, Bragdon MD, Lopez Rivera F, Biette... for career planning, it shouldn’t be used in isolation. Without clear dialogue between trainee and PI...Strategies to Resolve Conflict With Difficult Supervisors Management for Scientists: Seeking Feedback Management...

...Banskota, S., Davis, J. R., Newby, G. A., Chen, P. Z., Palczewski, K., & Liu, D. R. (2024). Engineered ...Nuñez, J. K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., ...problematic for low-abundance targets or natively isolated complexes. To address these limitations, the Funabiki...Funabiki Lab developed a technique called Magnetic Isolation and Concentration (MagIC)-cryo-EM that combines...

...imaging of voltage fluctuations. Image credit: Liu Z, et al. Cell. 2022.  Find JEDI-2p viral preps here...13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a) antibody is currently recommended for use...here!   Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-...

...278–282. https://doi.org/10.1038/s41586-021-03676-z  More resources on the Addgene blog Introduction ...Arbabi-Ghahroudi, 2017)!      Figure 1: Comparison of a heavy-chain only antibody and single-domain...