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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 54 results for: Z-ISO

Showing: 1 - 20 of 54 results

  1. CRISPR Guide

    Type
    Collection
    ... PMID: 23287718 Cui, Z., Tian, R., Huang, Z., Jin, Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo... Liu, Y., Gu, Z., Dickerson, K. E., Xie, S., Hon, G. C., Xuan, Z., Zhang, M. Q., Shao, Z., & Xu, J. (2017...Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee... 35236982 Bubeck, F., Hoffmann, M. D., Harteveld, Z., Aschenbrenner, S., Bietz, A., Waldhauer, M. C., ...H., Zhou, R., Lang, B., Meng, B., Weng, H., & Hu, Z. (2022). FrCas9 is a CRISPR/Cas9 system with high ... 24463574 Gao, S., Wang, Y., Qi, T., Wei, J., Hu, Z., Liu, J., Sun, S., Liu, H., & Wang, Y. (2023). Genome...Sun, N., Zeina, C. M., Gao, X., Rees, H. A., Lin, Z., & Liu, D. R. (2018). Evolved Cas9 variants with ...

  2. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JR, Aryee MJ, Joung JK...PMID: 25307932 Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O,... Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, Joung JK. 2016. High-fidelity CRISPR-Cas9 nucleases...: 24463574 Fujita T, Fujii H. 2013. Efficient isolation of specific genomic regions and identification...iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification...

  3. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...219–225 . https://doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova KS, Shmakov S, Koonin EV (2017..., Cress BF, Crits-Christoph A,  He C , Xu M, Zhou Z, Smock DC, Tang K, Owens TK, Krishnappa N, Sachdeva.../2020.07.17.209189v2 Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin EV, Zhang F ...Sun X, Wang Q, Xu J, Dong F, Yang S, Yang J, Zhang Z, Qian Y, Chen J, Zhang J, Liu Y, Tao R, Jiang Y, Yang...communities and into species not previously cultured or isolated (Rubin et al., 2020).  Figure 3: Multiplexed...

  5. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...Singh A, Kuksenko O, Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell ...collection that contains 1,836 genes encoded by 3,548 isoforms and two control vectors. The individual clones...purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that simplifies identification...identification of the transcription factor isoform in pooled screens. This collection and library can be...transcription factor open reading frames. Each isoform is uniquely barcoded with a 24 bp sequence, to ...

  6. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ... D, Huang C, Huff S, Hunker A, Johansen N, Juneau Z, Kalmbach B, Khem S, Kussick E, Kutsal R, Larsen R...Walker M, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, Ariza J, Dee N, Reding M, Ronellenfitch K...Goldy J, Cetin AH, Smith K, Way SW, Esposito L, Yao Z, Gradinaru V, Sunkin SM, Lein E, Levi BP, Ting JT,...CT Isocortex 230801 pAAV-AiE2575m-minBG-iCre(R297T)-BGHpA AiP20139 AiE2575m Cre Layer 6_CT Isocortex 214601... 6b Isocortex 220729 pAAV-AiE2586m-minBG-iCre(R297T)-BGHpA AiP2136 AiE2586m Cre Layer 6b Isocortex 230554... 6b Isocortex 220728 pAAV-AiE2587m-minBG-iCre(R297T)-BGHpA AiP2068 AiE2587m Cre Layer 6b Isocortex 163505...for cell populations in the following regions: Isocortex Hippocampal formation Cortical subplate Striatum...

  7. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...Resources References Radtke, A. J., Chu, C. J., Yaniv, Z., Yao, L., Marr, J., Beuschel, R. T., Ichise, H., ..., Gola, A., Thakur, N., Shih, R., Yao, L., Yaniv, Z. R., Beuschel, R. T., Kabat, J., Croteau, J., Davis...type, target species, target name, host organism, isotype, clone details, vendor, method, tissue preservation...with Addgene Antibodies 101: Choosing the Right Isotype...

  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Villette, V., Bradley, J., Wang, Z., Jiang, D., Zhang, G., … Lin, M. Z. (2024). A fast and responsive voltage... Find more neuroscience antibodies here! Wu, Z., Wang, W. (2024). Anti-APP [6E10-A5] in Immunohistochemistry...neurodegenerative diseases (Alzheimer’s disease or Hoarding disorder), but not in a patient with non-neurodegenerative...

  9. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...6624.2001 Han, Z., Luo, N., Ma, W., Liu, X., Cai, Y., Kou, J., Wang, J., Li, L., Peng, S., Xu, Z., Zhang, W...20616. https://doi.org/10.1074/jbc.M101559200 Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated.... https://doi.org/10.1016/j.ymthe.2006.05.009 Wu, Z., Miller, E., Agbandje-McKenna, M., & Samulski, R.... cells (Issa et al., 2023). AAV2 Despite being isolated second, AAV2 is the best studied and most commonly...

  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung ...Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR (2018) Evolved Cas9 variants with broad PAM...bacterial species Many more Cas9 orthologs have been isolated, and our understanding of their PAMs is shown ...same species. Expanding the CRISPR toolbox The isolation of novel CRISPR proteins has and will continue...

  11. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...doi.org/10.2144/000112585   Ding T, Huang C, Liang Z, Ma X, Wang N, Huo Y-X (2020) Reversed paired-gRNA.... https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger J, Plank C, Aneja MK, Rudolph C (2019) Segmented...recombination in samples. For a more detailed comparison of these cloning strains, browse the table of...preps. In some cases, we suggest gel extraction to isolate the full, intact plasmid. Please see our gel extraction...

  12. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...9-39 Gao Q, Xu W, Yan T, Fang X, Cao Q, Zhang Z, Ding Z, Wang Y, Wang X (2019) Rescue of a plant cytorhabdovirus....org/10.1104/pp.15.00636 Ma X, Zhang X, Liu H, Li Z (2020) Highly efficient DNA-free plant genome editing...often requiring several rounds of selection to isolate plants with the phenotype of interest. Genome engineering...

  13. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Gao, H., Bai, S., Li, C., Ding, R., Xue, N., Lei, Z., Chen, Y., Guan, Y., Siwko, S., … Li, D. (2023). ...., Miyabe, A., Araki, M., Hara, K. Y., Shimatani, Z., & Kondo, A. (2016). Targeted nucleotide editing ... 883–891. https://doi.org/10.1038/s41587-020-0453-z Thuronyi, B. W., Koblan, L. W., Levy, J. M., Yeh, ... G., Ma, X., Geng, H., Huang, W., Pang, X., Yang, Z., … Li, D. (2020). Dual base editor catalyzes both...favorably to other methods. In a head-to-head comparison with Cas9, Cas9 modified 9/12 known off-targets...

  14. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...-mEGFP AICSDP-63 mEGFP Alpha-actinin-2 Sarcomeric z-disks 124608 NPM1-mTagRFP-T AICSDP-69 mTagRFP-T Nucleophosmin...SLC25A17-mEGFP AICSDP-27 mEGFP Peroxisomal membrane protein PMP34 Peroxisomes 101785 TUBA1B-mTagRFP-T AICSDP...

  15. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...target in the parental cell line, but not in the iso-genic knockout control. This can involve cell surface... Schwartz, J., Kolahdouzan, M., Chen, C. X., You, Z., Benaliouad, F., Gileadi, O., McBride, H. M., Durcan.../doi.org/10.1111/bph.15467 Virk, H. S., Rekas, M. Z., Biddle, M. S., Wright, A. K. A., Sousa, J., Weston...

  16. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...share updated versions of the kit.” Reference Abil Z, Denard CA, Zhao H.  Modular assembly of designer ... protein that binds an alternate sequence. By comparison, Abil explained, the specificity of other RNA...

  17. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ...Paperwork. Vincent BJ, Scholes C, Staller MV, Wunderlich Z, Estrada J, Park J, Bragdon MD, Lopez Rivera F, Biette... for career planning, it shouldn’t be used in isolation. Without clear dialogue between trainee and PI...Strategies to Resolve Conflict With Difficult Supervisors Management for Scientists: Seeking Feedback Management...

  18. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...Banskota, S., Davis, J. R., Newby, G. A., Chen, P. Z., Palczewski, K., & Liu, D. R. (2024). Engineered ...Nuñez, J. K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., ...problematic for low-abundance targets or natively isolated complexes. To address these limitations, the Funabiki...Funabiki Lab developed a technique called Magnetic Isolation and Concentration (MagIC)-cryo-EM that combines...

  19. Hot Plasmids - October 2022

    Type
    Blog Post
    ...imaging of voltage fluctuations. Image credit: Liu Z, et al. Cell. 2022.  Find JEDI-2p viral preps here...13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a) antibody is currently recommended for use...here!   Evan GI, Lewis GK, Ramsay G, Bishop JM. Isolation of monoclonal antibodies specific for human c-...

  20. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...278–282. https://doi.org/10.1038/s41586-021-03676-z  More resources on the Addgene blog Introduction ...Arbabi-Ghahroudi, 2017)!      Figure 1: Comparison of a heavy-chain only antibody and single-domain...
Showing: 1 - 20 of 54 results