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Addgene
Showing: 1 - 12 of 12 results
  1. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ... functional A->G change. They fused ADAR deaminase domains (ADARDD) to dPspCas13b, but observed low RNA... editing, they employed hyperactive ADAR constructs, like ADAR2DD(E488Q). They also adjusted the structure...mutating residues in ADAR2 that interact with duplex RNA, they hoped to destabilize ADAR-RNA binding to decrease...Cas13 enzyme fused to an RNA adenosine deaminase (ADAR). Such a system would convert adenine to inosine...when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine if the template...editing portion of the protein, they examined ADAR1 and ADAR2, which deaminate adenosine to inosine in RNA...RNA. Using an ADAR2DD catalytic mutant, they showed that editing is mediated by ADAR2DD, not PspCas13b...
  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...catalytic domain of RNA editing enzyme, ADAR. This RBP-ADARcd fusion protein irreversibly edits adenine...
  3. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...members and get to know them! Khandan Baradaran, PhD Khandan Baradaran’s expertise in viral safety, gene therapy... with health authority regulations,” Baradaran says.  Baradaran’s path to science began in high school...new members to the Board of Directors: Khandan Baradaran, Frank Stegmeier, and Luk Vandenberghe. We’re ...in viral genetics. In regards to open science, Baradaran thinks the best way to solve problems is to work...think will get the research funded,” she says. Baradaran met Addgene Executive Director Joanne Kamens several...
  4. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ... On the 10th day of CRISPR, Addgene gave to me: RADARS from Gootenberg, Chen, and Abudayyeh🎵On the 9th...9th day of CRISPR, Addgene gave to me: RADAR from Gao🎵On the 8th day of CRISPR, Addgene gave to me: Functional...
  5. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...Cas13 (dCas13) with the adenine deaminase domain of ADAR2. Now, they’ve further developed this editor for ...editing by evolving the adenine deaminase domain of ADAR2 into a cytidine deaminase. This editor still retains...
Showing: 1 - 12 of 12 results