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We narrowed to 17 results for: adar

Showing: 1 - 17 of 17 results

  1. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ... functional A->G change. They fused ADAR deaminase domains (ADARDD) to dPspCas13b, but observed low RNA... editing, they employed hyperactive ADAR constructs, like ADAR2DD(E488Q). They also adjusted the structure...mutating residues in ADAR2 that interact with duplex RNA, they hoped to destabilize ADAR-RNA binding to decrease...Cas13 enzyme fused to an RNA adenosine deaminase (ADAR). Such a system would convert adenine to inosine...when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine if the template...editing portion of the protein, they examined ADAR1 and ADAR2, which deaminate adenosine to inosine in RNA...RNA. Using an ADAR2DD catalytic mutant, they showed that editing is mediated by ADAR2DD, not PspCas13b...

  3. CRISPR Guide

    Type
    Guide
    ...on a second ADAR variant that displays improved specificity, and editors carrying an ADAR truncation that...hyperactive adenosine deaminase that acts on RNA, ADAR2(E488Q) fused to catalytically dead Cas13b to create...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...catalytic domain of RNA editing enzyme, ADAR. This RBP-ADARcd fusion protein irreversibly edits adenine...

  5. CRISPR Guide

    Type
    Collection
    ...on a second ADAR variant that displays improved specificity, and editors carrying an ADAR truncation that...hyperactive adenosine deaminase that acts on RNA, ADAR2(E488Q) fused to catalytically dead Cas13b to create...

  6. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...members and get to know them! Khandan Baradaran, PhD Khandan Baradaran’s expertise in viral safety, gene therapy... with health authority regulations,” Baradaran says.  Baradaran’s path to science began in high school...new members to the Board of Directors: Khandan Baradaran, Frank Stegmeier, and Luk Vandenberghe. We’re ...in viral genetics. In regards to open science, Baradaran thinks the best way to solve problems is to work...think will get the research funded,” she says. Baradaran met Addgene Executive Director Joanne Kamens several...

  8. The 12 Days of CRISPR

    Type
    Blog Post
    ... On the 10th day of CRISPR, Addgene gave to me: RADARS from Gootenberg, Chen, and Abudayyeh🎵On the 9th...9th day of CRISPR, Addgene gave to me: RADAR from Gao🎵On the 8th day of CRISPR, Addgene gave to me: Functional...

  9. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...Cas13 (dCas13) with the adenine deaminase domain of ADAR2. Now, they’ve further developed this editor for ...editing by evolving the adenine deaminase domain of ADAR2 into a cytidine deaminase. This editor still retains...

  10. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...effects of ADAR2 by destabilizing its RNA binding. Editors carrying the delta-984-1090 ADAR2 truncation...rather than DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine deaminase to catalytically dead...

  16. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... to guanosine. RNA editors : Cas13 fused to the ADAR2 deaminase convert adenosine to inosine on RNA. Inosine...double-strand breaks. In mammalian cells, Cas13-ADAR2 does not require a specific flanking sequence on...
Showing: 1 - 17 of 17 results