...transduces BALB/cJ, CBA/J, and FVB/NJ mice, with minimal liver transduction observed in both BALB/cJ and ...enhanced transgene expression, compared to AAV9, in BALB/cJ mice but is more effective than AAV9 in many ...brain endothelial cells in C57BL/6J mice, but not in BALB/cJ mice, meaning that systemic capsids that rely...rely on LY6A interactions cannot cross the BBB in BALB/cJ mice (Hordeaux et al., 2018). This results in ... called LY6C1. This protein is expressed in both BALB/cJ mice and C57BL/6J mice (Shay et al., 2023; Huang... effectively transduces cells in the CNS of both BALB/cJ and C57BL/6J mice (Shay et al., 2023). AAV-PHP.AX... in transduction efficiency between C57BL/6J and BALB/cJ mice due to its LY6C1 dependence. MDV1A and MDV1B...

...following IV injection but fails to reach the CNS of BALB/cJ mice. These results were surprising because in...expression between the brains of C57BL/6J mice and BALB/cJ mice, suggesting it was a likely player in PHP.B...classic genetics approach and interbred C57BL/6J and BALB/cJ mice to map the inheritance pattern of PHP.B ...SNPs, PHP.B could bind it, but when LY6A contained BALB/cJ SNPs, binding was reduced. The Deverman group...permissive phenotype of mouse strains beside C57BL/6J and BALB/cJ, and the effects of overexpressed Ly6a on PHP.B...

...editors Nme2Cas9 CRISPR interference in Candida albicans E. coli genome-wide inhibition library To expand...AAV constructs CRISPR interference in Candida albicans A new CRISPR interference (CRIPSRi) platform for... for targeted gene repression in Candida albicans was developed by Rebecca Shapiro’s lab. After testing...repressed transcription of HSP90 an essential C. albicans gene. Read the paper in mSphere Find the dCas9...

...This post was contributed by guest bloggers Albert Cheng and Mark Wanner. CRISPR-Cas9 offers a leap forward...Casilio page. Many thanks to our guest bloggers! Albert Cheng, Ph.D., is an assistant professor at The ...andtranscriptome. You can follow him on Twitter @albertwcheng.   Mark Wanner (@markgenome on Twitter) is the...impact on clinicalprogress.   References 1. Cheng, Albert W., et al. "Casilio: a versatile CRISPR-Cas9-Pumilio...

... blurring the lines between science and art was Albert G. Richards, who passed away in 2008. His work ... Smithsonian Magazine, where the editor stated “Albert G. Richards, a teacher of dental radiography, had...especially those which are now extinct. A collection of Albert G. Richards’ work is held by The Museum of Jurassic...

... microbiology PhD student at the University of Alberta studying with the Szymanski lab. Reasons to study...which has recently moved from the University of Alberta in Edmonton, Canada, to the University of Georgia... microbiology PhD student at the University of Alberta studying with the Szymanski lab (though she’s tagged...

... justice.  Follow her on twitter @sung_vanessa. Albany, NY, USA 42.6526°N 73.7562°W – Nicholas Thompson...decided to go. At my local March for Science in Albany, NY, many tables were set up with various science...with some really great signs. The Congressman for Albany, Rep. Paul Tonko also came and spoke in support...

...includes pLX_305_Ovalbumin, a plasmid that enables introduction of the model antigen, Ovalbumin, into cell...

...this post from Erik Snapp, Associate Professor at Albert Einstein College of Medicine, to learn how problematic...Adaptive, Multitasking “CRISPR-OS” In this post, Albert Cheng and Mark Wanner from the Jackson Laboratory...

...Findlay, a Postdoctoral Fellow at the University of Alberta. If you’re like many researchers these days, you...currently a Postdoctoral Fellow at the University of Alberta. He is interested in everything molecular biology...

...have been well documented over the past 2 years (Alberts et al, PNAS 2014; Taylor, eLife, 2016). Several...Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing US biomedical research...

... (Tyr) gene in mouse zygotes, which results in albinism in mice. Of the eight mice born from Tyr Nanoblade... editing efficiencies of the chimeric and fully albino mice ranging from 11% to 100%, a level similar ...

...organisms, including bacteria, alternative fungi (C. albicans, pC2HB) (7) and mammalian cells. If the problem...two-hybrid system for the pathogenic fungus Candida albicans. Nucleic Acids Res. 2010;38(19):e184. Pubmed PMID...

...Health and he is currently an Associate Professor at Albert Einstein College of Medicine in the Department ...Costantini received her PhD in Biomedical Research from Albert Einstein College of Medicine. Her doctoral research...

...blogger Daria Shcherbakova, a faculty member at Albert Einstein College of Medicine. Several sets of near-infrared...blogger! Daria Shcherbakova is a faculty member at Albert Einstein College of Medicine. Her expertise is ...

...multiple copies of both vectors. Examples of use: Halbert et al., 2002 used an overlapping vector with 440...124–130 . https://doi.org/10.1038/sj.mt.6300322 Halbert CL, Allen JM, Miller AD (2002) Efficient mouse ...

...factors, consider using one of the alternative, albeit less ideal, verification approaches discussed in...generally sticky proteins such as bovine serum albumin. Secondary antibodies can also react with endogenous...

...CRISPR/Cas9   Erik L. Snapp, Associate Professor at Albert Einstein College of Medicine. Post: When is a Monomer...

... blogger Jessica Sacher from the University of Alberta and describes a technique you can use to find phage...

...Sacher, a microbiologist from the University of Alberta, saw that scientists were using Twitter to find...