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Showing: 1 - 20 of 79 results
  1. Immunocytochemistry

    Type
    Protocol
    ...6-diamidino-2-phenylindole (DAPI) Bovine serum albumin (BSA) Triton X-100 Primary antibody Secondary antibody...
  2. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-Calbindin [L109/39R] Calbindin Human Mouse IgG2a 177455 Anti-Calbindin [L109/57R] Calbindin Human...Human Mouse IgG2b 206635 Anti-CALB1/calbindin [L109/39R-2b] CALB1/calbindin Human Mouse IgG2b 206636 Anti-Homer1L...Human Mouse IgG1 206686 Anti-CALB1/calbindin [L109/39R-1] CALB1/calbindin Human Mouse IgG1 206689 Anti-VGAT...IgG2a 177459 Anti-Parvalbumin [L114/3R] Parvalbumin Rat Mouse IgG2a 177460 Anti-Parvalbumin [L114/81R] NPY...206641 Anti-CALB2/calretinin [L122/68R-2b] CALB2/calretinin Human Mouse IgG2b 206642 Anti-CALB2/calretinin... Mouse 182071 Calbindin scFv [L109/57] L109/57 Calbindin Human Mouse 182072 Parvalbumin scFv [L114/3] ...] L114/3 Parvalbumin Rat Mouse 182073 Parvalbumin scFv [L114/81] L114/81 Parvalbumin Rat Mouse 182075 ...
  3. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...transduces BALB/cJ, CBA/J, and FVB/NJ mice, with minimal liver transduction observed in both BALB/cJ and ...enhanced transgene expression, compared to AAV9, in BALB/cJ mice but is more effective than AAV9 in many ...brain endothelial cells in C57BL/6J mice, but not in BALB/cJ mice, meaning that systemic capsids that rely...rely on LY6A interactions cannot cross the BBB in BALB/cJ mice (Hordeaux et al., 2018). This results in ... called LY6C1. This protein is expressed in both BALB/cJ mice and C57BL/6J mice (Shay et al., 2023; Huang... effectively transduces cells in the CNS of both BALB/cJ and C57BL/6J mice (Shay et al., 2023). AAV-PHP.AX... in transduction efficiency between C57BL/6J and BALB/cJ mice due to its LY6C1 dependence. MDV1A and MDV1B...
  4. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    ...following IV injection but fails to reach the CNS of BALB/cJ mice. These results were surprising because in...expression between the brains of C57BL/6J mice and BALB/cJ mice, suggesting it was a likely player in PHP.B...classic genetics approach and interbred C57BL/6J and BALB/cJ mice to map the inheritance pattern of PHP.B ...SNPs, PHP.B could bind it, but when LY6A contained BALB/cJ SNPs, binding was reduced. The Deverman group...permissive phenotype of mouse strains beside C57BL/6J and BALB/cJ, and the effects of overexpressed Ly6a on PHP.B...
  5. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...editors Nme2Cas9 CRISPR interference in Candida albicans E. coli genome-wide inhibition library To expand...AAV constructs CRISPR interference in Candida albicans A new CRISPR interference (CRIPSRi) platform for... for targeted gene repression in Candida albicans was developed by Rebecca Shapiro’s lab. After testing...repressed transcription of HSP90 an essential C. albicans gene. Read the paper in mSphere Find the dCas9...
  6. Casilio: An Adaptive, Multitasking “CRISPR-OS”

    Type
    Blog Post
    ...This post was contributed by guest bloggers Albert Cheng and Mark Wanner. CRISPR-Cas9 offers a leap forward...Casilio page. Many thanks to our guest bloggers! Albert Cheng, Ph.D., is an assistant professor at The ...andtranscriptome. You can follow him on Twitter @albertwcheng.   Mark Wanner (@markgenome on Twitter) is the...impact on clinicalprogress.   References 1. Cheng, Albert W., et al. "Casilio: a versatile CRISPR-Cas9-Pumilio...
  7. Ras Pathway

    Type
    Collection
    ...proto-oncogene RAL RALA RALB v-ral simian leukemia viral oncogene homolog (ras related) RALBP1 RalA binding protein...downstream targets. Rajalingam K, Schreck R, Rapp UR, Albert S. Biochim Biophys Acta. 2007 Aug;1773(8):1177-...
  8. Science as Art

    Type
    Blog Post
    ... blurring the lines between science and art was Albert G. Richards, who passed away in 2008. His work ... Smithsonian Magazine, where the editor stated “Albert G. Richards, a teacher of dental radiography, had...especially those which are now extinct. A collection of Albert G. Richards’ work is held by The Museum of Jurassic...
  9. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... microbiology PhD student at the University of Alberta studying with the Szymanski lab. Reasons to study...which has recently moved from the University of Alberta in Edmonton, Canada, to the University of Georgia... microbiology PhD student at the University of Alberta studying with the Szymanski lab (though she’s tagged...
  10. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ... justice.  Follow her on twitter @sung_vanessa. Albany, NY, USA 42.6526°N 73.7562°W – Nicholas Thompson...decided to go. At my local March for Science in Albany, NY, many tables were set up with various science...with some really great signs. The Congressman for Albany, Rep. Paul Tonko also came and spoke in support...
  11. Cheng Lab CRISPR Casilio System

    Type
    Collection
    ...Casilio system from Albert Cheng's lab at Jackson Laboratories. Next generation CRISPR-Cas platform for... and References Casilio Plasmids Background The Albert Cheng and Haoyi Wang labs at The Jackson Laboratory...
  12. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...this post from Erik Snapp, Associate Professor at Albert Einstein College of Medicine, to learn how problematic...Adaptive, Multitasking “CRISPR-OS” In this post, Albert Cheng and Mark Wanner from the Jackson Laboratory...
  13. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...Findlay, a Postdoctoral Fellow at the University of Alberta. If you’re like many researchers these days, you...currently a Postdoctoral Fellow at the University of Alberta. He is interested in everything molecular biology...
  14. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...organisms, including bacteria, alternative fungi (C. albicans, pC2HB) (7) and mammalian cells. If the problem...two-hybrid system for the pathogenic fungus Candida albicans. Nucleic Acids Res. 2010;38(19):e184. Pubmed PMID...
  15. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...blogger Daria Shcherbakova, a faculty member at Albert Einstein College of Medicine. Several sets of near-infrared...blogger! Daria Shcherbakova is a faculty member at Albert Einstein College of Medicine. Her expertise is ...
Showing: 1 - 20 of 79 results