...Bargmann’s keynote speech and in the workshops on CRISPR techniques and new tools for conditional expression...of your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap...toolkit and plasmid assembly line for the creation of CRISPR targeting vectors that each contains a guide RNA...Schwartz and Jorgensen, 2016). Using SapTrap, this CRISPR plasmid can be created in a single tube. You can...Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans...Addgene blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids 101 blog...

...other CRISPR reporters Browse our CRISPR blog posts Resources on Addgene.org Browse the CRISPR guide...the genome, but they’re inefficient compared to CRISPR/Cas9 edits that rely on double strand DNA breaks...converted into BFP (blue fluorescent protein) upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et...Conversion: A Versatile Assay for the Quantification of CRISPR/Cas9-mediated Genome Editing. Molecular Therapy...guide Find CRISPR plasmids for your research ...

...choice. For more help with CRISPR, read our recent blog post explaining how CRISPR is being used for bacterial...Check out our Genome Engineering Overview and our CRISPR guide to find out how to use genome editing techniques...

...discovery. CRISPR is a great example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids...ubiquitin ligase, cellular senescence, autophagy, CRISPR, and so much more (find the full, searchable list...

... in applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L...Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development for scientists...

...editing Read more about CRISPR Learn all the basics about CRISPR in our CRISPR 101 blog series Resources... Cell Symposia: Gene- and Cell-based Therapies: CRISPR, Stem Cells, and Beyond meeting earlier this month...Addgene.org Find base editors for your research Read our CRISPR guide Find plasmids for your research ...

... Penn State Protein Ladder Engineered miniature CRISPR-Cas system for mammalian genome regulation and ...gene perturbation by: Gabrielle Clouse While many CRISPR-based genetic screening methods have primarily ...10.1038/s41598-021-96051-x Engineered miniature CRISPR-Cas system for mammalian genome regulation and ...and editing by: Rachel Leeson The large size of CRISPR-Cas effectors and fusion proteins, such as Cas9 and...engineering applications. CasMINI, a new miniature CRISPR-Cas system for editing and gene activation, is ...

...using the CRISPR-Cas9 system CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast...require custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used in immunology for in ...vitro screens of immune cell activity. However, a CRISPR-based tool for in vivo functional analysis of immune...Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes in any immune lineage...

...lentiviral inventory is based on CRISPR tools, though we do provide a few non-CRISPR related viral preps. Cas9...Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of choice after...can then be used both for screens (using pooled CRISPR knockout libraries) and individual gene knockouts... Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. PubMed...

...COVID-19: CRISPR-Based Detection Tools. The CRISPR Journal 3:142–145. https://doi.org/10.1089/crispr.2020.0030...post that highlights five different methods to use CRISPR to detect SARS-CoV-2. Other protocols and tools...more. Recently, scientists used the genome-wide CRISPR libraries, Brunello (knock out) and Calabrese (...Konermann S, Hsu PD (2021) Genome-wide, bidirectional CRISPR screens identify mucins as critical host factors...

...targeting range of CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam CRISPR/Cas9 is widely...2016).  The Kanemaki lab previously developed a CRISPR/Cas9 based method to tag endogenous proteins in...and other tags (Yesbolatova et al., 2019) using CRISPR. This toolkit makes it easy to tag your protein...

...Click on the links below to catch up on ways to use CRISPR in plants, to learn techniques for detecting genome...Posts from October 2016: Sequencing Options for CRISPR Genotyping Grad School Advice Part 2: Building ...Building Community Engineering the Plant Genome Using CRISPR/Cas9 Healthcare Consulting: A Door to the Business...

...Service, we’re distributing lentivirus (with many CRISPR tools included among the preps that are currently...lentiviral vectors in the repository are used to produce CRISPR tools, we’re starting the Viral Service with lentiviral... activators, Cas9 repressors, select gRNAs, and CRISPR pooled libraries. Whether you’d like to knockout... in experiments other than just those involving CRISPR and so we also have lentivirus for delivering control...

...and MECP2 protein, showing mutations corrected by CRISPR/Cas9 in wild-type human iPSCs. Image modified from... from Le et al., 2019. Gene editing via CRISPR/Cas9 and RNA-mediated correction of MECP2 mutant alleles...disease. Klaus Rajewsky’s group recently used the CRISPR/Cas9 system to both precisely insert the MECP2-...Keith Joung, PhD, and Feng Zhang, PhD, is pursuing CRISPR/Cas9 technology for precise correction of Rett ...to facilitate MECP2 curative strategies, such as CRISPR and RNA editing or the induction of iPSCs, are ...Rajewsky K, Chu VT (2019) Efficient and Precise CRISPR/Cas9-Mediated MECP2 Modifications in Human-Induced...

...PubMed. More CRISPR-Cas9 Resources: CRISPR 101: a blog series on the basics of CRISPR-Cas technology...technology CRISPR Resources from Addgene   ... crispr...

...Nature Methods. 2015 Aug 31. CRISPR-Display: A novel CRISPR technology for targeting RNA cargos...technique, called CRISPR-Display, that may help answer this difficult question. CRISPR-Display uses the...system provides users greater temporal control over CRISPR/Cas9 mediated genome modification and gene expression...139-42. doi: 10.1038/nbt.3149. Light-inducible CRISPR-Cas9 system for endogenous gene activation Optogenetics...Nucleases, the Gersbach lab has modified the RNA-guided CRISPR-Cas9 system to create a tool that is quick, versatile...robust. Dubbed the LACE system for light-activated CRISPR-Cas9 effector, Polstein and Gersbach fused the ...targeted to a very specific site on the genome via the CRISPR system. The ease and flexibility of the LACE technology...

... Based on CRISPR/Cas by Shravanti Suresh Guest blogger Shravanti Suresh who studies CRISPR at Iowa State...State University detailed five methods for using CRISPR to detect SARS-CoV-2, including many with plasmids...

...Resources at Addgene.org Check out our CRISPR resources and CRISPR for plants Bacterial Expression Systems...PZA-resistance by using different approaches, including CRISPR and recombination-mediated genetic engineering ...more information on bacterial engineering using CRISPR on our blog). Her research should provide us with...

...mixing and matching of parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning...use of Gibson assembly combined with the popular CRISPR technology and is described in a recent publication...second example of using Gibson Assembly along with CRISPR was also described recently (Jiang et al. 2015)...chromosome (up to 100 kb) were specifically cut out via CRISPR and then assembled into a vector using Gibson assembly...homology with the bacterial chromosome fragments. CRISPR cutting was used to circumvent the need to PCR ...Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9 nuclease cleavage combined with Gibson assembly...

...Susanna Bachle Figure 1: Initial steps in the CRISPR-pooled screening using sgRNAiBAR. Image from Zhu...'s lab at Peking University has developed a new CRISPR screening method that makes use of guide RNAs with...false-positive rate discovery rate, conventional CRISPR screens require a low multiplicity of infection...