Fluorescent Tagging of Endogenous Genes with SapTrap
Type
Blog Post
Published
Oct. 12, 2017, 2:26 p.m.
... other CRISPR/Cas systems used for endogenous protein tagging, please visit our website CRISPR/Cas Plasmids... of interest with a fluorescent protein fusion. CRISPR genome editing can help accomplish this goal. The...sequence (like GFP) can be inserted at the DSB. CRISPR has allowed scientists to tag and light up endogenous...better understand normal protein function. However, CRISPR protein tagging does have its limitations, especially...plasmid assembly toolkit called SapTrap to improve CRISPR genomic tagging in C. elegans. SapTrap offers scientists...systems, including mammalian cells.
CRISPaint (CRISPR-assisted insertion tagging), developed by the Hornung...interest to promote homology directed repair after a CRISPR/Cas9 induced break. Learn more about these constructs...