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We narrowed to 4 results for: doxycycline inducible cas9 expressing lentivirus

Showing: 1 - 4 of 4 results

  1. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...vectors can be used to express your gene of interest either constitutively or inducibly. If your gene of interest...needs to be expressed a certain point in the cell cycle, a transfer vector with an inducible promoter (... should be used. Inducible lentiviral transfer vectors The most widely used inducible lentiviral vector...derivative such as Doxycycline (Dox), the Tet-controlled transactivator (tTA, also expressed by your transfer...population of cells might express only the tTA or your transgene resulting in low inducibility and potentially ...Research Order Ready-to-Use Lentivirus Browse Our Lentivirus Guide Pages  ...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral...

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...capable of inducible expression of shRNA in target cells by the addition of tetracycline/doxycycline to the... with lentiCas9-Blast or with cell lines already expressing Cas9. Find lentiGuide-Puro. pSpCas9n(BB)-2A-GFP... Similar to pSpCas9(BB)-2A-Puro (PX458), pSpCas9n(BB)-2A-GFP (PX461) expresses both a Cas9 protein and...Find pMD2.G. pSpCas9(BB)-2A-GFP (PX458) - This plasmid from the Zhang lab expresses Cas9 from Streptococcus...and a gRNA. But instead of Cas9, this plasmid expresses Cas9n (a D10A nickase mutant) to nick, rather than...Addgene!) pX330-U6-Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has been widely popular in genome...DNA. Find pSpCas9n(BB)-2A-GFP (PX461). pSpCas9(BB)-2A-Puro (PX459) - This mammalian expression vector encodes...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...Kit contains Cas9, Cas9 nickase, and Cas9 activator expressing plasmids that can co-express 1-8 gRNAs. ...the Liu lab’s own pAc-sgRNA-Cas9 insect expression backbone, which expresses the gRNA from a Drosophila...parasites that constitutively express Cas9 with a library of sgRNA expression vectors (containing ten guides...CRISPR-Cas9-based tool for targeting CpG methylation by fusing catalytically inactive Cas9 (dCas9) with...cell lines expressing it. The Church lab fixed this issue by creating a new dCas9-VPR expression plasmid ...Furthermore, dCAS9-VPR expression is under the control of the TRE promoter, which allows for doxycycline-inducible...doxycycline-inducible expression. The end result is genomic integration of dCas9-VPR and controlled expression of the...

  4. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... purifying Cas9? Check out pET-28b-Cas9-His from Alex Schier's lab, designed for expression and purification... of Yonglong Chen, pCS2-3xFLAG-NLS-SpCas9-NLS is a Cas9 expression plasmid that was used by Chen and colleagues...colleagues, FokI-dCas9 expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain...stably expressing pTRE-Tight-MitoTimer and rtTA. The cells were pulsed with 2µg/ml of Doxycycline for 2...break. Next, the gRNA being tested is expressed along with Cas9. If the gRNA effectively cuts the target...Cre-based Brainbow-expressing cells. Autobow plasmids are all-in-one versions which express self-excising ...multiple host cells in order to optimize expression. The expression plasmids for a given species are based...
Showing: 1 - 4 of 4 results