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We narrowed to 25 results for: ena/1000

Showing: 1 - 20 of 25 results

  1. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ... but a select few are particularly special. Over 1000 of our 60,000+ deposited plasmids have been requested... UNC’s Bryan Roth says, Addgene has “literally enabled sharing with 100's of investigators worldwide. ...

  2. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...pieces of roughly equal size, typically between 200-1000 bp. This is to ensure that your downstream analysis...native proteins (so antibodies validated only for denatured proteins may not be a good choice) and the amount...

  3. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...two-photon imaging with newer lasers in the range of 1000–1080 nm. These industrial fiber lasers are becoming...lasers, with two-photon excitation at 1030 nm, enabling two-photon imaging without compromising on brightness...

  4. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...proximity ligation." Nature methods 3.12 (2006): 995-1000. PubMed PMID: 17072308.  4. Dieck, Tom, et al. "...segment is labeled with fluorescent probes that enable visualization of the location of the protein-protein...

  5. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ... (response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target...expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very strong, also called TDH3 ... expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length version is...

  6. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...used to calculate the viral titer: GC/mL = {[(R*C)(1000/V)]*D} R = Reaction volume C = Copies/uL V = Volume...Increasing the elongation time to 2 minutes and the denaturation time to 1 minute has been shown to increase ...

  7. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    Published
    July 29, 2025, 1:15 p.m.
    ...proteins are quite large — comprising approximately 1000–1400 amino acids — which poses a challenge for efficient...systems. Importantly, they allow transient delivery, enabling transgene-free genome editing (no transgene integration... used to simultaneously target multiple genes, enabling efficient multiplexed genome editing in a single... diverse plant and animal systems, potentially enabling more precise and accessible genome engineering...

  8. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...of template should have homology arms between 500-1000 bps. HDR efficiency with circular plasmid templates... the repair of breaks that lack a second end to enable DSBR or SDSA.   Homology directed repair and genome...

  9. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...Low 572/ 610 FP595 Tetramer Green Dark/ Red 70 - 1000 Medium 590/ 600   Choosing a photoactivatable...irreversible and reversible photoactivation - and have enabled sophisticated imaging techniques.     Irreversible...Martin, et al. "Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... E. coli.” ACS Synthetic Biology 4(9) (2015):987-1000. PubMed PMID: 25822415. Find plasmids from this...plasmids. Each destination vector contains GFP, enabling you to select cells with high GFP expression. ...This kit is built for serious multiplexing and enables users to express up to 7 gRNAs. The kit contains...

  12. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...produces point mutations at a rate of up to 1 per 500-1000 bp. In comparison, the DNA replication error rate...lentiviral CRISPR library     CRISPR pooled libraries enable researchers to screen the entire genome for genes...way scientists create new traits in bacteria and enabled the in-depth study of many biological processes...only genome engineering method in bacteria that enables rapid, automated, and high-throughput genome editing...temperature-controlled expression of the MutL mutant enables transient suppression of DNA repair during oligonucleotide...developed CRISPR tools for epigenome editing that enable the precise study of particular epigenetic modifications....1-GoldenGate, pcDNA3.1-GoldenGate-VP64 Maria-Elena Torres-Padilla pTALYM3, pTALYM4 Boris Greber pTAL7a...

  13. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...and flow cytometry. F1000Research, 13, 817. https://doi.org/10.12688/f1000research.154034.1 Laflamme, ...interest. If you are working with cells that are amenable to genetic knockout, this represents a very robust...essential to the cell’s function, it likely is not amenable to knockout approaches. In these cases, we would...synaptotagmin 1 page. The data produced is available on the F1000 gateway and Zenodo community.  Knockdown approach...be difficult to achieve sufficient knockdown to enable robust assessment of antibody selectivity. Additionally...). Scaling of an antibody validation procedure enables quantification of antibody performance in major...Schwartz, J., Kolahdouzan, M., Chen, C. X., You, Z., Benaliouad, F., Gileadi, O., McBride, H. M., Durcan, T. ...

  14. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ... organization issues – F1000 just announced the launch of their F1000Workspace.  Give your favorite searching...where I dump all of my papers and they all have filenames (year first author name). It’s rudimentary, but...Maaike Pols, PhD, a Developmental Editor at F1000Research  ...

  15. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...integrate permanently into the host genome and thereby enable long-term stable gene expression. Gamma-retroviral.... This empty MuLV vector from the Weinberg lab enables researchers to express siRNAs of their choice in...Plasmid Kit Retroviral Barcoding Library (Kit # 1000000097) The Winslow lab barcoding library consists of...Multiple Lentiviral Expression System Kit (Kit #1000000060) The MuLE (Multiple Lentiviral Expression) system...

  16. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...you want to have 100x-1000x coverage of your library: in other words, 100-1000x more reads than number...proper use requires careful amplification and maintenance of good representation. Addgene wishes you luck...

  17. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...Potential. PLoS Genet 5:e1000683 . https://doi.org/10.1371/journal.pgen.1000683 Bétermier M, Bertrand P...journal.pgen.1004086 Ghezraoui H, Piganeau M, Renouf B, Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson...

  18. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...Research in Neurobiology, 4, 100086. https://doi.org/10.1016/j.crneur.2023.100086. Challis, R. C., Ravindra...antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing (Chen et al., 2023). AAV-BI30, developed...S., Meltzer, J. C., Buzhdygan, T., Cheng, M. J., Sena-Esteves, M., Bennett, R. E., Sullivan, T. P., Razmpour...by engineering capsid–receptor interactions that enable crossing of the blood–brain barrier. PLoS Biology...carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral...Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery across species...

  19. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...Kamenyeva et al, 2015. Cellular trafficking studies enabled by fluorescent proteins The optimization of GFP...live-cell imaging (intravital imaging) studies it’s enabled are revealing the cellular behaviors that mediate...antigen presenting cells, but two-photon microscopy enabled scientists to see how chaotic and random the scanning...of fluorescent pathogens and fluorescent cells enables scientists to monitor cell interactions in diverse...proteins are excited by 750 nm to 760 nm laser light, enabling dual color imaging studies with blue or cyan proteins...photoactivable or photoconvertible proteins, as it enables tracking throughout the lifetime of the cell.     ...2009): 6379-6393. PubMed PMID: 19414791. 7. John, Beena, et al. "Dynamic imaging of CD8+ T cells and dendritic...

  20. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...such as BenchSci and FPbase are working hard to enable researchers by making it easier to find information...it easier for researchers to share data thereby enabling you to do modified analyses on published data .... Finally there are new publishing outlets like F1000, PeerJ, PLoS, and bioRxiv that make it easier to...
Showing: 1 - 20 of 25 results