Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
Type
Blog Post
Published
Sept. 18, 2018, 1:33 p.m.
...ACE) in real-time. The reporter constitutively expresses eGFP to indicate reporter abundance, and mCherry...and N177F mutations). Its excitation maximum is reported as 603 nm, while the emission maximum is 675 nm...
ACE system: A versatile in vivo fluorescent reporter for rapid quantification of base-editing and Cas9...editing tools, the Reuben Harris lab has deposited a reporter plasmid that allows for rapid fluorescence-based... out a frame-shift mutation within the mCherry reporter. Quantification of editing efficiencies simply...containing rat APOBEC1. Finally, they show that the ACE reporter can also quantify Cas9 cleavage, thus exhibiting...editing activities.
Click here to find the ACE reporter plasmid!
Martin et al., Nucleic Acids Research...