... 6.8 (red) 2.8/4.1 7.1 h (red) pFast-FT-N1 pTRE-Fast-FTpBAD/HisB-Fast-FT Medium-FT 401 (blue), 579...produced the three separate timers shown in the table: Fast-FT, Medium-FT, and Slow-FT. These display progressively...Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue), 583 (red) 466 (blue), 606 (red) ...

...affinity for glutamate but slower kinetics; v857 has faster kinetics but lower affinity) and with a variety...two-photon microscopy. The resulting JEDI-2P has faster response kinetics and increased brightness, sensitivity... Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell...transfer at the subcellular level requires a probe with fast kinetics and high signal/noise ratio. Here, Takeharu...Figure 7: B-gTEMP temperature-sensitive probe enables fast measurement of temperature and local heat transfer...

...captured in a single camera exposure. This is much faster than relying on raster scanning as is done in confocal...characteristic to capture in these types of samples is their fast movements. Depending on the sample, a temporal resolution...sample is then collected and projected back to a fast, sensitive detector such as a scientific CMOS camera...planes of a sample may also be useful for providing fast imaging along with optical sectioning (5). One such...preferred technique for many of these samples due to its fast temporal resolution and decreased phototoxicity....

...increasingly faster kinetics. The opsin ChETA, for example, is a ChR2 opsin mutated to have faster off kinetics...kinetics and allow neurons to repolarize faster.  Chemogenetics does not offer the same level of control...design.   Optogenetics Chemogenetics Timing Fast activation/deactivation Prolonged activation Targeting...

...that I'm glad we're so fast and affordable. Something in their world should be fast and affordable. 4. Stem...after the OSKM paper – as a prime example of the fast movement in this field.  As an Addgene scientist...engineering technologies, stem cell techniques are neither fast nor inexpensive.  After talking to numerous poster...

...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery at the...you to our guest bloggers, Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor at the University... Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an Associate Professor at the University of...Miller School of Medicine. Find plasmids from the Tsoulfas lab here.     References: H. Siedentopf, and ...

...Aligning two nucleotide sequences is probably the fastest BLAST search to perform and will save you time ...Highly similar sequences (megablast)”, which is very fast and works best when the identity between your sequence... [Our QC process would be trouble-free and much faster if 95% of our results were always correct!]  Since...base pair from your results. This option is not as fast as megablast, but can return longer alignments to...

... The pEcCas plasmid can be operated at 37 ℃ for faster growth of its E. coli host, and the addition of...industrial strain. Not only is MUCICAT better, it is faster. Using MUCICAT only needs 2 newly constructed plasmids...the MUCICAT system has the following advantages: Fast and site-specific multicopy chromosomal integration...on developing microbial genome editing tools for faster construction or reprogramming of cells to understand...

...blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field, requiring people who are willing to... tasks, and then executing on them in a timely fashion while allowing for creative solutions to emerge...some of the more challenging parts of the job? With fast turnover of projects, pressure to maintain consistently...case, consulting can sometimes be challenging. The fast paced nature of projects often means working long...

...Sadowski 1985). The FLP system works in a similar fashion to the Cre system. The FLP recombinase recognizes...inversion, and translocation of DNA in a similar fashion as between loxP sites (For details see Cre-lox ...Michel is a Postdoctoral Researcher at MIT and is fascinated by the question of how genes and proteins coordinate...9661200.  3. Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking Cells Induces Gamma Rhythm and Controls ...

...perform:      Fig. 2: Quick-stepping to a FASTA file   With our outline of individual steps ...could iterate our development and testing cycle faster due to the ease of jumping back and forth between... For example, our bbduk process created trimmed FASTQ files as output, which we specified as input to ...could adjust the compute resources when needed, for faster runtime and decreased cost compared to purchasing...

...coli multiplexing plasmid is available from the Koffas lab. Scaling up your multiplexed gRNAs with Golden... on a separate plasmid. Multiplexing in E. coli Koffas Lab CRISPathBrick Multiplex Plasmid: This system... E. coli CRMAGE Plasmids: The CRMAGE system is a fast, multiplexable method that combines CRISPR and recombineering-based...possible with efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time needed...

...with a confocal microscope, yet I was once again fascinated by the world that reveals itself when looking...by the ASM Agar Art Winners! It is absolutely fascinating to see the living art designed by creative minds...steady hand. By connecting art and science, a fascinating interface is created that produces loads of inspiration...

...your preference, we are here to make it fast and easy. Fast Approval: The UBMTA used by Addgene is ...approvals, so your plasmid requests can get to your lab faster. For Everybody: Friendly Support: Have a question...

...example, the far-red light-activated split-Cas9 (FAST) system constitutively expresses the C-terminal ...surface of skin and is less phototoxic. Using the FAST system, Ye’s lab could edit a tdTomato fluorescent...in mice using Minicircle DNA vectors encoding the FAST constructs. This proof-of-concept model opens intriguing... Alter chromatin architecture. Yu et al., 2020 FAST BphS from R. sphaeroides Split Cas9 Genome editing...

...is activated eight times faster than GRABNE and deactivated three times faster. This enhanced response ...sensitive to and selective for norepinephrine and faster to respond than GRABNE. While GRABNE is blocked...

...to the grant officers at Fast Grants. We received a miraculous grant from Fast Grants, which was approved...coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that the majority of deposited materials...

...ordering, making it a toss-up as to whether it’s faster to order or try to make it in your lab (but if ... you need a lot of plasmids, it’s almost always faster to order from us!) Ask the PI If you want a plasmid...working at speed or money if budget is a concern. The faster methods, like Gibson assembly, are typically more...

...amino acid peptide, the CRY2clust domain enables fast and reversible oligomerization when fused to a protein...termination of light stimulation. CRY2clust enables faster assembly and disassembly dynamics, higher sensitivity...optogenetic system (OptoSTIM1), caused two-fold faster changes in intracellular Ca2+ levels than the original...

...Kinetics How fast is the process you would like to monitor? Neurons, for example, have unusually fast calcium...three GCaMP types based on their kinetics: GCaMPf (fast) GCaMPm (medium), GCaMPs (sensitive but slower)....