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Addgene
Showing: 41 - 60 of 216 results
  1. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...amino acid peptide, the CRY2clust domain enables fast and reversible oligomerization when fused to a protein...termination of light stimulation. CRY2clust enables faster assembly and disassembly dynamics, higher sensitivity...optogenetic system (OptoSTIM1), caused two-fold faster changes in intracellular Ca2+ levels than the original...
  2. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...determined by their homology. You might also consider FastCloning, first described by Chaokun Li and colleagues...the prices of the additional enzymes required. FastCloning may be the least expensive of all the options...Addgene!). References 1. Li, Chaokun, et al. "FastCloning: a highly simplified, purification-free, sequence-and...
  3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... 100% negative prediction rate. It is also the fastest test that has been developed so far.  A similar...challenging samples as in Two-Step-SHERLOCK or in a fast, high-throughput manner in one step as One-Pot-SHERLOCK.../2020.04.07.028167 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst...
  4. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...stimulate at a fast frequency without missing an action potential, then a channel with fast kinetics may...V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast optogenetic control. Nature Neuroscience, 13(3...
  5. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... require high sequencing read counts making them fast and viable options for multiplex sequencing in many... for all DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially... (e.g. dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive...
  6. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...accolades that would launch our careers in science. Fast forward nearly 20 years and I am known by some colleagues...in Jefferson’s case, even a lot about everything. Fast forward a few hundred years and there are currently... will not only get your paper out the door much faster, but getting helpful feedback from other people...
  7. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ... of CRISPR/Cas9 technology are coming along at a fast and furious pace. In December, as we've blogged ...it before, but CRISPRs allow research to move so fast." On the very same day the monkey story hit the ...
  8. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...researchers worldwide. “COVID-19 has spread incredibly fast and without any notion of national boundaries. To...policies and ultimately treatments in a timely fashion.”  With this in mind, Bourque and Tara Moriarty...
  9. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ... (e.g., 100 nm) of DA. In addition, they exhibit fast kinetics (10 ms on and 100 ms off), enabling detection...PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging of dopamine dynamics with designed...
  10. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...imaging of voltages. This includes monitoring of fast bursts, as well as slow up/down voltage states in... Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy. Cell...
  11. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...a simple egg is packed with information. I am fascinated with how developmental programs unfold, absorbing...comparative anatomy showed me that some of the most fascinating biology is found in weird and rare organisms ...
  12. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...easy to manipulate, it’s affordable, and it grows fast to boot. The yeast strains S. cerevisiae and S. ...to culture and easy to work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic...
  13. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...more about one another. They also provided both breakfast, lunch, and snacks. At the end of the workshop...experiences like workshops at Lab Central. Because of the fast pace of work in the Addgene lab, we don’t often ...
  14. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...Scientists need fast access to the tools required to study SARS-CoV-2, but with many academic research... the scientific community. Addgene was awarded a Fast Grant to ensure that the scientific community will...
  15. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...schedule might work. If not, perhaps a 3 hour breakfast once a month. All participants must commit to ...each other practice meeting people – compete for fastest to set up a coffee date or most cards collected...
  16. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...spatiotemporal resolution for in vivo work. APEX2, a faster peroxidase-based labeling approach, is an alternative...30-fold enrichment compared to E. coli’s BirA*. Faster labeling kinetics also means that BASU could potentially...
Showing: 41 - 60 of 216 results