...network of the participants Can be a lot of fun! Form your own peer mentoring group In my travels speaking...My advice has been to find 5 interested peers and form your own peer mentoring circle. In a Peer Mentoring...Center and Brandeis University. 5 easy steps to forming your own peer mentoring group Find 5-8 colleagues..., the group mentoring format could work wonders.  I have now seen the formation of hundreds of mentoring...mentees who meet together for an 8 month formal program. After the formal program ends, some 50% of these groups...supporters and advisors? I tried to start a more formal mentoring program at my company, but there weren't...mentees. So I experimented with a group mentoring format where 1 mentor met with a group of mentees to get...

...ways: 1. Fill out our "Suggest a Plasmid" form Fill out the form with the name of the specific plasmids ...

...with the tracrRNA forms the scaffold portion of a gRNA and the “spacer region” forms the target sequence...protein. Upon target binding, Cas9 undergoes a conformational change that positions the nuclease domains ...

... recruited proteins involved in DNA synthesis to form a stable DNA-protein complex that will continue ...the ORI permit binding of regulatory proteins that form a DNA-protein complex that recruits a helicase which...between active and inactive DnaA levels. The active form of DnaA necessary for initiating replication upon...replication at the origin. The initiation complex formed by the action of IHF and the RepA-DNA-DnaA complex... from the ORI. A DNA polymerase completes the formation of each initiation complex and synthesizes the...

...delivery techniques may hold the key. In the simplest form, translating gene therapies from conceptual design...slight variations in their viral capsids. Novel forms of AAV capsids are discovered frequently and recent...technology have enabled us to generate new and refined forms of capsids with improved specificity. AAV vectors...study showed significant clinical efficacy against a form of blindness known as Leber’s congenital amurosis...

...We’re big fans of all forms of research sharing and with Open Data Day 2020 coming up on March 7th, what...input your data for sharing on the site. The entry form has 5 general question types and it takes approximately...all, we’d appreciate you sharing it through this form. In honor of Open Data Day we’d like to give a shoutout...launched this pilot in 2019 as an open science platform for scientists using Addgene AAV viral preps to...handy?  Contact info: Give us your contact information so we can follow up with you if we have any questions...injection. How long post-injection did you wait until performing the experiment? What detection methods did you...

...constructs, why not let us know? Fill out our referral form and we’ll take care of the rest. Know of a useful...collection? Tell us about it with our Suggest a Plasmid Form! Finally, if you would like to get the word out...deposit or email [email protected] for more information or to start a deposit. If you have a colleague...

...site. Four proteins (TniQ, Cas8, Cas7, Cas6) that forms the QCascade DNA-targeting module together with ... The requirement for multiple Cas proteins to form the Cascade targeting complex is a hallmark of Type... or have its own nuclease activity. Instead, it forms a novel co-complex with the transposition protein... using RNA-DNA base pairing. Separately, TnsABC forms a transposase complex with the mini-transposon, ...single-plasmid INTEGRATE (pSPIN), which offer simple transformations for smaller cargos, and the ability to easily...Clone desired DNA cargo into the mini-transposon Transform plasmid(s) into cells and plate overnight Screen...

...creative. However, if we think of creativity as a form of innovation, it’s clear that creativity is absolutely...company-wide New Ideas Survey. Anyone could fill out the form at any time and a member of our Business Development...in charge of implementing it. The purpose of this form was to encourage the sharing of ideas, not to give...out on the open sea Creativity comes in many forms. Whether you can paint like Frida Kahlo or you’re...PhDs found that many researchers feel they lack formal training in a variety of transferable skills. At...References Grant, A. (2017). Originals how non-conformists move the world. Penguin Publishing Group. Day...

...right? RNA performs almost all of its biological functions in our cells in the single strand form, but double...within the R-loop that is now vulnerable to various forms of DNA damage, and these structures can in turn ...in several autoimmune disorders, including some forms of lupus and rheumatoid arthritis. These types ..., why it’s important for cells to sense their formation, and how clinicians, researchers, and even farmers...Still others use dsDNA to store their genetic information. All of these viruses have one thing in common...genomic regions are prone to persistent R-loop formation, presumably due to their abundance of R-loops ...or mechanisms which suppress endogenous dsRNA formation are dysregulated, immune responses can mount in...

...most difficult form, which makes it the perfect transferable skill. Almost every other form of presenting...will have to learn this skill in its most difficult form. You’re presenting complex data, to a group of peers...goal.   As a scientist, public speaking isn't a performance art. You’re (usually) presenting either to share...public speaking gig. Being able to give a clear, informative presentation, speak at conferences, or even just...

... pathway, and then cleaved to generate an active form of the protein. To monitor autophagy, the Pho8Δ60... Pho8Δ60 localizes to the cytosol in an inactive form unless autophagy is induced. Then non-selective ...weight shift of Pho8 from its uncleaved to cleaved form is the final read out of this assay. This is also... provide information about the contents of the autophagosome nor does it provide information about what...

...Practice your presentation skills (as much as you can), form a peer mentoring group to get real feedback Write...peer mentoring group? "What Makes a Good Mentor?" Form Your Own Peer Mentoring Group: A How-To Guide for... create and stick to timelines Be a mentor in a formal program Start something (a journal, a science lunch...

...AAV1, AAV5, and AAV retrograde (the most popular form), this viral vector is used to deliver DNA encoding...-FRT. Additionally, you could use the retrograde form to specifically express Cre in neurons with axons...

...neutral (protonated) and anionic (deprotonated) forms of the chromophore which contribute to the 2 peaks...chromophore photoconverts predominantly to the anionic form, causing increased fluorescence when excited with... the chromophore from the neutral to the anionic form, which allows PA-GFP to exhibit up to a 100-fold...violet light, both variants are photoconverted to forms that emit brighter red fluorescence. Since emission...another monomeric PA-FP which unlike mEosFP, can form it’s chromophore at a more comfortable 37°C (5)....scientists later developed red fluorescent PA-FPs by performing several rounds of random mutagenesis screening...are preferred over the monomeric one since the formation of mEosFP’s chromophore requires a temperature...

...interplay of these two ideas he said “science is a form of art to me… The problem is that scientists sometimes...of conservation.” Appreciating science as an art form taps into an emotional response within the viewer...They can study molecules, analyze terabytes of information on black screens, but not to know even the life...

... which is available in both liquid and powdered forms. Due to improper handling or poor lab techniques...techniques, mycoplasma can spread through this form of powdered media. Filtration does not ensure complete sterilization...fluorescent microscope, the mycoplasma DNA appears in the form of small clusters, apart from the cellular DNA. ...apart from mycoplasma. Always have a backup in the form of cryopreserved vials of the cell lines even though...laminar airflow before and while you are working. Perform routine checks for mycoplasma contamination, before...the problem of contamination through aerosols. Perform culturing without the use of antibiotics since ...

...derivatives of all three germ layers, form embryoid bodies, and form teratomas [11]. iPSCs also possess ...ESCs are pluripotent stem cells that are able to form all adult cell types and are obtained from the inner...afterwards for ESC research purposes, with the informed consent of the donor [7]. Adult stem cells (ASCs... somatic cell conversion: conversion via iPSC formation, commonly referred to as reprogramming and, direct...

...titer: (Plaque Forming Units (PFU) or Infectious Units (IU), per mL)  Plaque formation assay: permissive... is further protected by histone-like proteins forming the nucleocapsid. B) Three generations of adenoviral...counted and titer is calculated in terms of plaque forming units (pfu), taking initial vector dilution into...presence of RCA in the viral stock by doing a plaque formation assay on A549 cells . Alternatively, antibody-...correct recombinant pAdV plasmid is identified, re-transform into a bacterial strain not prone to recombination...

...protein (probably while it is being synthesized) can form a complex with an ssDNA guide. Therefore, researchers...fraction of nascent NgAgo protein molecules manages to form a functional complex with ssDNA leading to inefficient...NgAgo (or a novel endonuclease) protein that can form a complex with a 5’-P-ssDNA guide at room temperature...necessarily represent the views of Addgene. Addgene performs Sanger sequencing on select regions of all distributed...plasmids as part of quality control, but does not perform functional tests. The newest genome engineer sharing...researchers were having difficulty achieving indel formation (gene disruption) with this new technology. To...respondent out of 88 confirmed successful indel formation at a target site. Also, 41 respondents attempted...