No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
Type
Blog Post
Published
June 18, 2020, 1:15 p.m.
...chains and two small light chains that stick out to form the “arms” holding the highly variable regions that...directly provide the three sybody libraries in the form of mRNA for academic research.
The template plasmids...ribosome to pause at the end of the mRNA molecule, forming a ribosome complex consisting of the translated...For more information read our secondary nanobodies blog post.
A rapid selection platform for generating...weeks (Zimmermann et al., 2020). This selection platform was designed so that any standard lab can quickly...proteins.
The full protocol for this sybody selection platform can be found here and relevant plasmids are available... Synthetic single domain antibodies for the conformational trapping of membrane proteins. eLife 7: . https...