An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
Type
Blog Post
Published
Dec. 17, 2020, 1:30 p.m.
...drug resistance (Chen et al., 2013), fluorescence (GFP; Tzur et al., 2013), or rescue of mutant phenotypes...homology arms to knock in large epitopes, such as GFP (Paix et al., 2014). A further development of this...efficiencies, obtaining as many as 100 independent GFP knock-ins from a single injected animal.
PAM sequence...