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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 41 results for: hyper

Showing: 1 - 20 of 41 results

  4. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...Others include: pHluorin & pHTomato (pH sensors), HyPer (H2O2 sensor), ArcLight (voltage sensor), and iGluSnFr...

  6. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...mobile DNA, called a transposon, that’s a bit hyperactive and likes to jump around from one location in... at rates similar to retroviral vectors. The hyperactive version of the SB transposase, SB100X, is ~100...transcription start sites, CpG islands, and DNaseI hypersensitivity sites. Like Sleeping Beauty, piggyBac has ...human cells similar to retroviral vectors. The hyperactive PB transposase (hyPB) is ~10-fold more active...transcription start sites, CpG islands, and DNaseI hypersensitivity sites. Tol2 is only active in vertebrates ...

  7. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...treated with sucrose or other osmolytes to induce hypertonicity display enhanced monoclonal antibody production...Shen, 2011). To our knowledge the effect of hyperosmolarity on AAV production yields had yet to be examined... and antibody production under conditions of hyperosmotic stress." Advances in animal cell biology and...18597326. 5. Shen, Chun Fang, and Amine Kamen. "Hyperosmotic pressure on HEK 293 cells during the growth ...

  9. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...constructs Anti-GFP [N86/8R] ready-to-use antibody Hyperfolder YFP CRISPR-Csm: A novel RNA targeting system...eLife.43322. PMID: 30667360; PMCID: PMC6377228. Hyperfolder YFP- a chemically stable tool for microscopy ... The Liu and Petsko labs recently engineered hyperfolder YFP (hfYFP), a FP well-suited for the demands...

  10. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts resulted in...Mates L, et al. Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable...

  12. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...Community Relations Director for Curiosity Collider (hyperlink https://curiositycollider.org/) , which she co-founded...and the Outreach Manager for Science Borealis (hyperlink http://scienceborealis.ca/)    ...

  13. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...autosomal dominant mutation in MYBPC3 that leads to hypertrophic cardiomyopathy. An adult male with a heterozygous...applicable in many situations. In the case of hypertrophic cardiomyopathy and other genetic disorders, ...

  14. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...cation channel) light-activated channels to hyperpolarize tumor cells, which resulted in tumor regression...activation, it stays open for a longer duration. Hyperpolarizing chloride channel SwiChr++ - A channelrhodopsin...

  15. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    .... https://pubmed.ncbi.nlm.nih.gov/24390935/ Hypercompact genome editor CRISPR-Casɸ By Melina Fan The ...toolbox with their discovery of CRISPR-Casɸ, a hypercompact genome editor from the Biggiephage clade of ...

  16. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...photoactivatable, optogenetic accuators to depolarize or hyperpolarize membranes. Traditional FRET reporters could ...depolarization increases the BRET signal whereas hyperpolarization decreases the signal. The authors additionally...

  17. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...results in efflux of chloride ions, subsequent hyperpolarization, and inhibition of action potential generation...spectrum as HR and also results in membrane hyperpolarization but unlike HR, this is achieved through hydrogen...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...binding sites, which then recruit an MS2-fused hyperactive variant of activation-induced cytidine deaminase...deaminase (AID). AID normally mediates somatic hypermutation to generate diverse antibodies; in the CRISPR-X...

  19. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ... gave to me: efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs. On the sixth...
Showing: 1 - 20 of 41 results