...multiplexed gRNAs and of dCas9 fusions. The dCas-KRAB activator and the dCas-VPR repressor are introduced...contain an EGFP reporter. Expression of the dCas9-KRAB activator or dCas9-VPR repressor in hPSCs is also... piggyBac gRNA delivery and the all-in-one dCas9-KRAB and dCas9-VPR targeting plasmids can be found at...

...cause cell lethality. The Krüppel associated box (KRAB) is a naturally occurring transcriptional repressor...frequently fused to Cas9 for this purpose. dCas9-KRAB plasmids for repressing target genes in a variety... Cas9 repressors include dCas9 alone and a dCas9-KRAB fusion, both targeted to the promoter region. Cas9...

...we are now adopting dCas9, dCas9-VP64, and dCas9-KRAB in our system,” Sakuma said. “These are sure to ...

...VP64 or VPR activates transcription, whereas dCas9-KRAB fusions repress transcription. Both of these methods...frequently inherited by daughter cells. For example, KRAB induced silencing was transient and quickly reversed...

...AAVS1-integrated, inducible, and fluorescent dCas9-KRAB and dCas9-VPR for quantifying and tracking cells...

...repression of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts!  Turn it on Turn...

...is then tethered to a repressive domain, such as KRAB, to prevent efficient transcription from sgRNA targets...

...activators (such as dCas9-VP64) or repressors (dCas9-KRAB or dCas9-SRDX) can also be used to activate or repress...