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Showing: 1 - 13 of 13 results

  1. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence...construct. Plasmids 175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572...Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene #110821 ) VPR ( Addgene #63798) ...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ...tagged insert options rtTA On Root 11651 pLVUT-tTR-KRAB Lentiviral vector for inducible expression of transgene...information about cloning and additional plasmids tetR-KRAB On Aebischer & Trono 16542 pBI-MCS-EGFP Expression...

  4. Plan Your Experiment

    Type
    Collection
    ...such as dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements of target gene dCas9-KRAB is more effective...

  5. CRISPR Plasmids - Yeast

    Type
    Collection
    ...fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with...

  6. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with...

  8. CRISPR Plasmids - Plants

    Type
    Collection
    ...fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with...

  9. Lentivirus Plasmids

    Type
    Collection
    ...tet-responsive H1 promoter Trono 11651 pLVUT-tTR-KRAB 3rd Inducible expression of transgene or shRNA. ...

  11. CRISPR Guide

    Type
    Collection
    ...transcriptional activator (e.g. VP64) or repressor (e.g. KRAB; Figure 9A). Other examples of activation systems...Weissman labs , is an all-in-one dCas9 fusion with KRAB, DNMT3A, and DNMT3L. CRISPRoff maintains gene silencing...
Showing: 1 - 13 of 13 results