...multiplexed gRNAs and of dCas9 fusions. The dCas-KRAB activator and the dCas-VPR repressor are introduced...contain an EGFP reporter. Expression of the dCas9-KRAB activator or dCas9-VPR repressor in hPSCs is also... piggyBac gRNA delivery and the all-in-one dCas9-KRAB and dCas9-VPR targeting plasmids can be found at...

...cause cell lethality. The Krüppel associated box (KRAB) is a naturally occurring transcriptional repressor...frequently fused to Cas9 for this purpose. dCas9-KRAB plasmids for repressing target genes in a variety... Cas9 repressors include dCas9 alone and a dCas9-KRAB fusion, both targeted to the promoter region. Cas9...

...we are now adopting dCas9, dCas9-VP64, and dCas9-KRAB in our system,” Sakuma said. “These are sure to ...

...VP64 or VPR activates transcription, whereas dCas9-KRAB fusions repress transcription. Both of these methods...frequently inherited by daughter cells. For example, KRAB induced silencing was transient and quickly reversed...

...AAVS1-integrated, inducible, and fluorescent dCas9-KRAB and dCas9-VPR for quantifying and tracking cells...

...repression of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts!  Turn it on Turn...

...is then tethered to a repressive domain, such as KRAB, to prevent efficient transcription from sgRNA targets...

...cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence...construct. Plasmids 175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572...Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene #110821 ) VPR ( Addgene #63798) ...

...activators (such as dCas9-VP64) or repressors (dCas9-KRAB or dCas9-SRDX) can also be used to activate or repress...

...fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with...

...tagged insert options rtTA On Root 11651 pLVUT-tTR-KRAB Lentiviral vector for inducible expression of transgene...information about cloning and additional plasmids tetR-KRAB On Aebischer & Trono 16542 pBI-MCS-EGFP Expression...

...such as dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements of target gene dCas9-KRAB is more effective...

...cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg), this would be...

...dilutions of the lentiviral vector pHAGE EF1α dCas9-KRAB . The following day, cells were treated with puromycin...

...fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with...

...such as dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements of target gene dCas9-KRAB is more effective...

...fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with...

...fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with...

...fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with...

...tet-responsive H1 promoter Trono 11651 pLVUT-tTR-KRAB 3rd Inducible expression of transgene or shRNA. ...