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We narrowed to 3 results for: lentiviral based polycistronic vector

Showing: 1 - 3 of 3 results

  1. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...safe for clinical applications.     Lentiviral: Lentiviral vectors infect dividing and nondividing cells...reprogramming vectors available from Addgene. Another drawback is that lentiviral vectors insert in different...8 kb, so either a single polycistronic vector or multiple single-gene vectors can be used to package reprogramming...Non-integrating iPSC delivery methods Viral Vectors Adenovirus: Adenoviral vectors infect dividing and nondividing...methylation. Another downside of using EBNA1 based vectors is that the plasmid can hang around for awhile...Transgene Expression Mediated by a Sendai Virus-Based Vector Using a Nonstructural C Protein and Endogenous...

  2. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...Multiple Lentiviral Expression Systems (MuLE) Kit: This kit can be used to create lentiviral vectors expressing...“Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.” Nucleic Acids Research...into distinct expression vectors using the enzyme BbsI. These expression vectors each contain Type IIS restriction...Cas9 or dCas9-containing destination vectors. Destination vector options include humanized wt Cas9, dCas9...wt hCas9 entry vectors are supplied with the kit, you can clone your own entry vectors containing other... inserted into a Cas9-containing destination vector based on pCAMBIA. Cas9 is plant-optimized, and plasmids...containing vector using Golden Gate assembly, and up to 8 gRNAs may be expressed simultaneously. Vectors for ...

  3. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...create a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ...expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT vector ensures...complex polycistronic lentiviruses. The MuLE toolbox contains a series of building-block vectors that are...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...combinations of genetic elements in a single lentiviral vector provides a powerful tool to modify mammalian...routes in various conditions. The plasmids and lentiviral vectors encoding these proteins can also be used ...
Showing: 1 - 3 of 3 results