Viral Production at Addgene
Type
Blog Post
Published
Oct. 24, 2023, 1:15 p.m.
...for in our reported titers.
Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...protocols.
Browse our AAV vector collection.
Lentivirus
Production
Lentiviral preparations ("-LV” catalog... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...Concentrated lentiviral preparations (“-LVC” catalog items) are generated from lentiviral preparations...