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We narrowed to 3 results for: lentiviral shrna expression vector plko 1 puro

Showing: 1 - 3 of 3 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab...TRC cloning vector. Tet-pLKO-puro - Tet-pLKO-puro contains all the necessary elements for packaging, reverse...3rd generation lentiviral vector that contains a scrambled shRNA. This scrambled shRNA is used as a negative...Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This plasmid expresses the S. pyogenes CRISPR chimeric...Addgene’s pLKO.1 protocol. Pro-tip: this plasmid grows more slowly than standard plasmids. Find pLKO.1 - TRC... with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such as our second ...BB)-2A-Puro (PX459) V2.0 - This plasmid from the Zhang lab is a mammalian vector that expresses both SpCas9...

  2. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    Published
    Jan. 8, 2014, 3:10 p.m.
    ...backbones for shRNA expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -...continued to see requests grow for lentiviral packaging and expression vectors, including pMD2.G and psPAX2....Fire's set of vectors for C. elegans research was our most requested item of 2005. shRNA - In 2006, we...you're familiar with the pLKO plasmids you'll note that those are lentiviral constructs. In 2007 we continued...TOPFlash, scramble shRNA for use as a negative control, and empty backbones such as pBABE-puro for retroviral...retroviral expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs - Genome engineering...

  3. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...compound of interest. Lentiviral vectors Popular Lentiviral Transfer Vectors    Expressing your gene of interest...generation lentiviral vector backbone from the Shih lab. You can find more lentiviral expression vectors (empty...and expressing your gene of interest with GFP expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning... handling and expression of these vectors when used in experiments. Retroviral vectors make up a large...Additionally, retroviral vectors can regulate gene expression by delivering shRNA or miRNA. The ever expanding...
Showing: 1 - 3 of 3 results