... 7 gRNAs into one destination vector, making multiplexing easy.  Disadvantages of Golden Gate cloning...Addgene’s restriction cloning guide! CRISPR 101: Multiplex Expression of gRNAs Choosing a Molecular Cloning...

...binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your calcium reading with ...you need to free up crowded optical space for multiplexing, GECO sensors are available in green, blue, ...

...counts making them fast and viable options for multiplex sequencing in many laboratories. Nevertheless,...DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially toxic...dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive to...

...detection (14-20). LDR has also been extended to multiplexed probe sets that allow the simultaneous interrogation...extends earlier studies through a high-sensitivity multiplexed format (21, 27). This methodology has been used...: PMC148763. 15. Pingle, Maneesh R., et al. "Multiplexed identification of blood-borne bacterial pathogens...

...vectors. In 2017, Zetsche et al. developed a Cpf1 multiplexing approach using a single crRNA array to express...publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex Gene Editing by CRISPR–Cpf1 Using a Single crRNA...

...cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously produces ... Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on Addgene.org...

... they have developed this further to provide multiplexed detection, higher sensitivity, and more. Read...

...Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing...PMC5972399. 12. Molla, K., Shih, J. & Yang, Y. Multiplex CRISPR-mediated base editing in rice. In Genome...

...Stefan Stricker’s lab, is a single step gRNA multiplexing system that generates vectors containing high...

...benefits of this technique. It is even possible to multiplex ultrasound dyes because these dyes “collapse” ...

...Kellner MJ, Joung J, Collins JJ, Zhang F (2018) Multiplexed and portable nucleic acid detection platform ...(C2c2) Learn more about Cas12a (Cpf1) and its multiplex genome editing abilities Resources on Addgene.org...

...protein purification through simple and efficient multiplexing. pCri plasmids are divided into two sets, each...

...arg2 ultrasound signals. This also means that multiplexing is possible if bacteria are sequentially exposed...

...both colors allows for increased options for multiplexing in experiments, including ones that study alpha2...

...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife 8:e43322. https...

...CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics." bioRxiv (2019): 335547...library-specific identifiers act like a timestamp and help demultiplex the scRNA-seq data and build multi-generational...

...Central PMCID: PMC4364232. Ma, Hanhui et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...

.... The lab demonstrated that XMAS-TREE can be multiplexed and can be used in human pluripotent stem cells...

...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Elife. 2019 Jan 22;8:...

...www.neil-t.com/ .   References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." ...