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We narrowed to 8 results for: p16

Showing: 1 - 8 of 8 results
  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... TetR with the transcriptional activation domain VP16 taken from the herpes simplex virus. tTA binds to... created by fusing CymR to the activation domain VP16. cTA binds to promoters containing putative operator...Fusing the LexA DNA-binding domain (DBD) to GAL4 or VP16 activation domain results in chimeric GAL80-repressible...Kingsbury RC, McKnight SL. Functional dissection of VP16, the trans-activator of herpes simplex virus immediate...
  2. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...activation domain such as VP16 to dCas9 or the recruitment of multiple copies of VP16 termed the “Sun Tag” ... only two constructs (the other containing dCas9-VP16). Like CRISPRi, CRISPRa is only effective when sgRNAs...
  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    .... By replacing wild type SOX2 with either a SOX2-VP16 (strong activator) or SOX2-HP1 (strong repressor...repressor) fusion protein, the authors show that the VP16 transcription activation domain fusion increases the...
  4. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...first request was on September 8, 2004 (for pcDNA-p160MBP, if you're curious.) Since then, we've distributed...
  5. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request for a kit came on March...
Showing: 1 - 8 of 8 results