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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 13 results for: p16

Showing: 1 - 13 of 13 results

  1. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... TetR with the transcriptional activation domain VP16 taken from the herpes simplex virus. tTA binds to... created by fusing CymR to the activation domain VP16. cTA binds to promoters containing putative operator...Fusing the LexA DNA-binding domain (DBD) to GAL4 or VP16 activation domain results in chimeric GAL80-repressible...Kingsbury RC, McKnight SL. Functional dissection of VP16, the trans-activator of herpes simplex virus immediate...

  3. 15 Hot Plasmids from 2017

    Type
    Blog Post
    .... By replacing wild type SOX2 with either a SOX2-VP16 (strong activator) or SOX2-HP1 (strong repressor...repressor) fusion protein, the authors show that the VP16 transcription activation domain fusion increases the...

  4. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...first request was on September 8, 2004 (for pcDNA-p160MBP, if you're curious.) Since then, we've distributed...

  7. Addgene: The First Twenty Years

    Type
    Blog Post
    ...plasmid request came on September 8, 2004, for pcDNA-p160MBP, while the first request for a kit came on March...

  9. Tetracycline Inducible Expression

    Type
    Collection
    ...tetracycline-controlled transactivator (fusion of TetR with VP16 transcriptional activation domain); rtTA: reverse...tetracycline-controlled transactivator (fusion of mutated TetR with VP16 transcriptional activation domain). For simplicity...the activation domain of the herpes simplex virus VP16 protein (Gossen and Bujard, 1992). This work also...absence of tetracycline, tTA binds to the TRE and its VP16 domain promotes gene expression. In the presence...transactivator (rtTA) was created by fusing rTetR with VP16. In the absence of tetracycline, rtTA is unable ...three copies of a minimal activation domain from VP16 for increased induction of gene expression and reduced...

  10. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Isocortex 214518 pAAV-AiE2366m-minBG-SYFP2-WPRE3-BGHpA AiP1691 AiE2366m SYFP2 Lamp5 interneurons Isocortex 220737...formation 214506 pAAV-AiE2333m-minBG-SYFP2-WPRE3-BGHpA AiP1653 AiE2333m SYFP2 CA3 pyramidal neurons Hippocampal...

  11. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Mammalian BsaI none S. pyogenes Huang pAC154-dual-dCas9VP160-sgExpression 48240 Mammalian BbsI yes, activate...pMCS-rybozyme-IRES-CAS9 64668 Mammalian yes, cut S. pyogenes W Fujii MSP1673 65769 Bacteria T7 yes, cut N. meningitidis Joung...

  12. TALEN Plasmids and Kits

    Type
    Collection
    ...four repeats of the minimal activation domain of VP16) at the C terminus. 47389 pcDNA3.1-GoldenGate-VP64...
Showing: 1 - 13 of 13 results