Tetracycline Inducible Expression
Type
Collection
...tetracycline-controlled transactivator (fusion of TetR with VP16 transcriptional activation domain); rtTA: reverse...tetracycline-controlled transactivator (fusion of mutated TetR with VP16 transcriptional activation domain). For simplicity...the activation domain of the herpes simplex virus VP16 protein (Gossen and Bujard, 1992). This work also...absence of tetracycline, tTA binds to the TRE and its VP16 domain promotes gene expression. In the presence...transactivator (rtTA) was created by fusing rTetR with VP16. In the absence of tetracycline, rtTA is unable ...three copies of a minimal activation domain from VP16 for increased induction of gene expression and reduced...