Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
Type
Blog Post
Published
July 26, 2018, 12:46 p.m.
...less phosphatase-accessible.
Kinase Translocation Reporters (KTRs)
Kinase Translocation Reporters (KTRs...fluorescent kinase reporters. A KTR is comprised of a fluorescently-tagged substrate for a kinase of interest...proxy for kinase activity.
Advantages of KTRs
When compared to FRET reporters for measuring kinase activity...Kinases: they regulate many proteins, with ~1/3 of human proteins predicted to be phosphorylated on at...for regulating signal transduction and measuring kinase activity at the single-cell level can aid in drawing...phenotype. One method for monitoring live single-cell kinase activity is FRET, but FRET reporters are challenging...Covert Lab provides an alternative tool with their Kinase Translocation Reporters (KTRs) whose cellular localization...