...optimized vectors to highlight a key advantage of CRISPRa over traditional cDNA overexpression: isoform-specific...expressed genes between Bdnf I-overexpressing neurons and Bdnf IV-overexpressing neurons. Among the upregulated...including transgenic mice, neuron-optimized viral vectors, and high-throughput screening approaches. Here...largely precludes delivery by adeno-associated vectors (AAV). This issue was recently circumvented by ...by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH) transgenic mouse (Zhou et al., 2018). This...activator that recruits up to twenty copies of the p65-HSF1 transcriptional activator to each genome-bound...sgRNAs can be found here. Neuron-optimized viral vectors Alternatively, Cas9 activators can be delivered...

...tracrRNA was combined with the sgRNA expression vector allowing the screen to be conducted with only two... a sgRNA with two MS2 RNA aptamers, and the  MS2-P65-HSF1 helper protein (Konermann et al., 2015). Root...screens performed with open reading frame (ORF) overexpression libraries for resistance to MEK inhibition....inhibition. Although both Calabrese and the ORF overexpression library identified several overlapping genes, most...

...expression vector which gives close to 100% cloning efficiency and gene expression. The pUCXKT vector ensures...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with ...custom-cloned vectors in a Multisite Gateway reaction with a desired destination vector expressing a separate...a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with the...MXS-chaining kit contains 5 empty chaining vectors plus a destination vector and a collection of building blocks...binding protein (a portion of CRY2) fused to the p65 activation domain, which activates transcription ...below. Scientists can obtain dCas9-trCIB1, CRY2PHR-p65, and other plasmids from this article through Addgene...