...This construct, built from the pBAD33 backbone, contains BLADE, the PBAD promoter, and a mCherry reporter...plasmid. It does not include the PBAD promoter. If you already have a pBAD33-based plasmid expressing your...AraC protein that controls transcription from the PBAD promoter. “AraC is widely used in synthetic biology...of AraC using a mCherry reporter downstream of a PBAD promoter. While none of the constructs gave as strong...reporter downstream of the PBAD promoter. To use this plasmid with your gene of interest, you would replace... a previously cloned gene of interest, under the pBAD promoter, can be controlled with light without the...expressing superfolder GFP instead of mCherry under the PBAD promoter. They created a bacterial lawn on an agar...

... expression vectors. Negative inducible promoter pBad is another popular prokaryotic promoter often used...regulatory protein AraC binds O and I1 sites upstream of pBad, blocking transcription. The addition of arabinose... media with glucose decreases cAMP and represses pBad, decreasing promoter leakiness.   Other examples...promoters, pLac is known to be slightly leaky, and pBad is likely a better option since expression can be...

...antibiotics, resulting in the plasmid pBAD-bARGSer-AxeTxe (Fig. 1a).  With pBAD-bARGSer-AxeTxe, gas vesicle expression...systems in E. coli. The arabinose-inducible promoter pBAD and a low-copy number origin of replication (p15A...Expressing bARGSer in E. coli. (a) Plasmid diagram of pBAD-bARGSer-AxeTxe. (b) Methods to assess bARGSer expression...

...2.7/4.7 3.9 h (red) pMedium-FT-N1 pTRE-Medium-FT pBAD/HisB-Medium-FT Slow-FT 402 (blue), 583 (red) ... 2.6/4.6 28 h (red) pSlow-FT-N1 pTRE-Slow-FT pBAD/HisB-Slow-FT -  Applications of fluorescent ...red) 2.8/4.1 7.1 h (red) pFast-FT-N1 pTRE-Fast-FTpBAD/HisB-Fast-FT Medium-FT 401 (blue), 579 (red)...

...bacterial expression vectors are inducible (e.g. pBAD LIC cloning vector (8A)) and have an epitope tag...

...bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein expression from each...

...the influence of structure on an FP’s properties. pBAD-LSSmCherry1 is a long Stokes shift variant, which... two-photon microscopy using Ti-Sapphire lasers. pBAD-RDSmCherry1 is a red-shifted variant which, with...

...the anti-inducer fucose Weaker. Commonly found in pBAD vectors. Good for rapid regulation and low basal...

...downstream of the arabinose (Ara)-inducible promoter (PBAD), and the A118 gene downstream of the diacetylphloroglucinol...

...-inducible lac promoter, the arabinose-inducible pBAD promoter and the endogenous phage pL promoter.  ...