Sequencing Primers
Type
Guide
...signal, 5' of MCS in pBABE vectors, forward primer pBAD Forward ATGCCATAGCATTTTTATCC (Invitrogen) For vectors...vectors with E. coli araBAD promoter, forward primer pBAD Reverse GATTTAATCTGTATCAGG (Invitrogen) For vectors...Invitrogen) 3' of MCS in pTrcHis vector, same as pBAD-R, reverse primer Puro-F GCAACCTCCCCTTCTACGAGC 3...