Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 6 results for: renilla luciferase constructs

Showing: 1 - 6 of 6 results

  1. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...Nano-lanterns contain a Renilla luciferase variant fused to an FP; when supplied with a luciferase substrate, the... problems of low luminescence By itself, Renilla luciferase has a low quantum yield, and when transiently...first Nano-lantern, a fusion of a mutagenized Renilla luciferase (RLuc8) with Venus. This luminescent technology...energy transfer (BRET). Photons emitted by a Renilla luciferase variant are used to excite the fused fluorescent...fluorescent imaging! These eNL constructs use the brightest characterized luciferase, NanoLuc, and its substrate...the luciferase transfers energy to the FP, resulting in a fluorescent signal. Since their first publication...need for an excitation light source. Instead, a luciferase substrate like coelenterazine is supplied in ...

  2. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...expressing luciferase Luciferase reporter assays for monitoring gene regulation Since firefly luciferase was ...Well-established Natural and engineered luciferases Although firefly luciferase was the first bioluminescent reporter...remains popular, other luciferases have been identified in the sea pansy (Renilla reniformis), click beetle...have engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...Oplophorus gracilirostris) luciferase and engineered to be extra bright. Other luciferases have been modified ...mammalian systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties...element is cloned upstream of the luciferase gene, and expression of luciferase is measured via the light produced...

  3. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...than our more common luciferases like firefly (FLuc) and Renilla (RLuc) luciferase. This is important not...coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce...codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from the ... creation of NanoLuc® Luciferase from a rather dull 19kDa Oplophorus luciferase by Promega’s Advanced ...systems. This construct is available through Addgene (Plasmid # 87127). Pairing NanoLuc® luciferase with fluorescent...lab, if you read a paper and saw an interesting construct you wished to use, you could either make it yourself...Addgene makes it so much easier to share your constructs from lab to lab. Promega supports the Addgene...

  4. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...Administration of Substrate: Some luciferase reporters, such as Renilla luciferase (RLuc) or NanoLuc, require...bacterial reporters that use fluorophores and luciferases to track bacteria in real time, but each of these...different bacteria, Bourdeau et al created two constructs optimized for expression in E. coli: acoustic...acoustic collapse to aid in the development of ARG constructs with new acoustic phenotypes. Want to start using...Blog Learn more about in vivo imaging tools The luciferase reporter gene The green fluorescent protein reporter...vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent protein biosensors ...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation of its substrate...CMV-TO) expression, or a promoterless construct. Pre-constructed Entry vectors containing Cas9, EGFP, ...of inducible promoter constructs. Up to 34 building blocks were used to construct 20kb long inserts. The...expression from the reporter construct. The sgRNA targeting Gaussia luciferase can be easily replaced with...transfer (BRET), whereby photons emitted by a Renilla luciferase variant are used to excite a fused fluorescent...culture media as a chemical substrate for Renilla luciferase. This approach is especially suited to complement...tag carried on all constructs.   pXPG: An alternative to the widely used luciferase reporter gene plasmid...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...with Protein A-Renilla luciferase and the “prey” proteins are tagged with Firefly luciferase. The bait and...Howarth lab created the MBPx-SpyCatcher construct. This construct is used to produce a maltose binding protein...However, the laborious construction of repair templates and guide RNA constructs, as well as the difficult... Human Kinase Domain Constructs Kit, a library of human kinase domain constructs developed through a large-scale... that results in the creation of many E. coli constructs with greatly varied levels of expression. The...can be used to easily create protein expression constructs fused with epitopes or fluorophores, generate...platform for generating both simple and complex DNA constructs Have you ever wished you could design a gene...
Showing: 1 - 6 of 6 results