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Showing: 21 - 40 of 53 results

  1. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ... by delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel... Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors 101: Chemogenetics...

  3. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons...use viral prep in serotype AAV5.  Get the channelrhodopsin here Astrocyte specific tight affinity glutamate...

  5. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...Activation of these neurons via the channel Channelrhodopsin 2 (ChR2) reduced glutamate transmission from...mouse to deliver Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified receptor protein that...

  7. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...Veenman et al., 1992) or fluorescent molecules like rhodamine-isothiocyanate (RITC; Thanos et al., 1987) are...Vidal-Sanz M., and Aguayo A.J. (1987). The use of rhodamine-B-isothiocyanate (RITC) as an anterograde and ...

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...simultaneously measuring the activities of CaMKII and RhoA during induction of structural plasticity in single...expression plasmids, FRET constructs, and CaMKII and RhoA sensors. Laviv, et al. Nat Methods. 2016. PubMed...

  9. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...report describes a functional vector such as a channelrhodopsin Images showing the injection site as well ...

  12. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...be inserted into the genome using CRISPR/Cas forHomologous Recombination (HR)? How long should the homology...

  17. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...spectrum. I recently presented Kurt Vonnegut’s Slaughterhouse-Five, and the responses ranged from “one of...

  19. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...Cas9’. The Cas9 nuclease is a genome engineering powerhouse, and for good reason. It is guided by an RNA ...
Showing: 21 - 40 of 53 results